Mechanisms of leukotriene E4 partial agonist activity at leukotriene D4 receptors in differentiated U-937 cells

• Published in: The Journal of biological chemistry Vol. 264; no. 33; pp. 19845 - 19855
• Main Authors: D L Saussy, Jr, H M Sarau, J J Foley, S Mong, S T Crooke
• Format: Journal Article
• Language: English
• Published: Bethesda, MD American Society for Biochemistry and Molecular Biology 25.11.1989
• Subjects: Aminoacid receptors (glycine, glutamate, gaba); Benzofurans; Biological and medical sciences; Calcium - metabolism; Cell Differentiation; Cell Line; Cell receptors; Cell structures and functions; Fluorescent Dyes; Fundamental and applied biological sciences. Psychology; Fura-2; Humans; Kinetics; Leukotriene E4; Manganese - metabolism; Manganese - pharmacology; Models, Theoretical; Molecular and cellular biology; Receptors, Immunologic - metabolism; Receptors, Leukotriene; Spectrometry, Fluorescence; SRS-A - metabolism; SRS-A - pharmacology; Arachidonic acid derivatives; Biological receptor

Leukotriene E4 (LTE4) is shown to be a partial agonist of leukotriene D4 (LTD4) in differentiated U-937 cells. The data that support this conclusion are: 1) LTE4 completely displaced [3H]LTD4 from its receptors in U-937 cell membranes. 2) LTE4 induced only 30 +/- 4% of the maximal Ca2+ transient ind...