Application of an Integrated Omics Approach for Identifying Host Proteins That Interact With Odontoglossum ringspot virus Capsid Protein

• Published in: Molecular plant-microbe interactions Vol. 28; no. 6; pp. 711 - 726
• Main Authors: Lin, Pin-Chun, Hu, Wen-Chi, Lee, Shu-Chuan, Chen, Ying-Lan, Lee, Chi-Ying, Chen, Yet-Ran, Liu, Li-Yu Daisy, Chen, Po-Yen, Lin, Shih-Shun, Chang, Ya-Chun
• Format: Journal Article
• Language: English
• Published: United States The American Phytopathological Society 01.06.2015
• Subjects: alanine; Amino Acid Sequence; Capsid Proteins - genetics; Capsid Proteins - metabolism; cell division; coat proteins; Computational Biology; gel chromatography; gene silencing; genes; Genomics; Glutamic Acid; high-throughput nucleotide sequencing; immunity; leaves; mass spectrometry; Models, Molecular; Molecular Sequence Data; mutants; Nicotiana - genetics; Nicotiana - metabolism; Nicotiana - virology; Nicotiana benthamiana; Odontoglossum ringspot virus; pathogenicity; Plant Diseases - virology; plant diseases and disorders; Plant Immunity; Plant Leaves - virology; Plant Proteins - genetics; Plant Proteins - metabolism; plant viruses; Protein Interaction Mapping; proteinase inhibitors; proteomics; Recombinant Fusion Proteins; reverse transcriptase polymerase chain reaction; Sequence Alignment; Sequence Analysis, DNA; tobacco; Tobamovirus - genetics; Tobamovirus - metabolism; transcription (genetics); Transcriptome; transmission electron microscopy; virion; viruses

The glutamic acid at position 100 (E 100 ) in the capsid protein (CP) of Odontoglossum ringspot virus (ORSV) plays an important role in long-distance viral movement in Nicotiana benthamiana. The ORSV E100A mutant, which has a glutamic acid to alanine substitution, shows a loss of systemic infectivit...