Role of Cellular Actin in the Gene Expression and Morphogenesis of Human Respiratory Syncytial Virus

• Published in: Virology (New York, N.Y.) Vol. 252; no. 1; pp. 137 - 148
• Main Authors: Burke, Emily, Dupuy, Lesley, Wall, Cynthia, Barik, Sailen
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 05.12.1998
• Subjects: actin; Actin Cytoskeleton - chemistry; Actin Cytoskeleton - metabolism; Actins - immunology; Actins - isolation & purification; Actins - physiology; Animals; Cell Line; Chickens; Chromatography, Affinity; Cytochalasin D - metabolism; Cytoskeletal Proteins - analysis; cytoskeleton; Gene Expression Regulation, Viral; Goats; Humans; Mice; microfilament; Paramyxovirus; Protein Conformation; Rabbits; Recombinant Proteins - metabolism; respiratory syncytial virus; Respiratory Syncytial Viruses - genetics; Respiratory Syncytial Viruses - growth & development; transcription; Transcription, Genetic; Transcriptional Activation; Virion - chemistry; microfilament; actin; transcription; cytoskeleton; respiratory syncytial virus; Paramyxovirus

Cytoskeletal protein actin and nonactin cellular proteins were essential for human respiratory syncytial virus (RSV) gene expression.In vitro,specific antibodies against actin inhibited RSV transcription, whereas antibodies against other cytoskeletal proteins had little or no effect. Affinity purifi...