Artificial control of gene expression in mammalian cells by modulating RNA interference through aptamer–small molecule interaction

• Published in: RNA (Cambridge) Vol. 12; no. 5; pp. 710 - 716
• Main Authors: An, Chung-Il, Trinh, Vu B., Yokobayashi, Yohei
• Format: Journal Article
• Language: English
• Published: United States Cold Spring Harbor Laboratory Press 01.05.2006
• Subjects: Aptamers, Nucleotide - metabolism; Base Sequence; Cell Line; DEAD-box RNA Helicases; Dose-Response Relationship, Drug; Endoribonucleases - genetics; Endoribonucleases - metabolism; Gene Expression Regulation; Gene Silencing; Humans; Molecular Sequence Data; Nucleic Acid Conformation; Phosphodiesterase Inhibitors - pharmacology; Recombinant Proteins - metabolism; Ribonuclease III; RNA Helicases - genetics; RNA Helicases - metabolism; RNA Interference - drug effects; RNA, Small Interfering - chemistry; RNA, Small Interfering - genetics; RNA, Small Interfering - isolation & purification; RNA, Small Interfering - metabolism; Theophylline - pharmacology; Transfection

Recent studies have uncovered extensive presence and functions of small noncoding RNAs in gene regulation in eukaryotes. In particular, RNA interference (RNAi) has been the subject of significant investigations for its unique role in post-transcriptional gene regulation and utility as a tool for art...