A Novel Quantitative Mass Spectrometry Platform for Determining Protein O-GlcNAcylation Dynamics

• Published in: Molecular & cellular proteomics Vol. 15; no. 7; pp. 2462 - 2475
• Main Authors: Wang, Xiaoshi, Yuan, Zuo-Fei, Fan, Jing, Karch, Kelly R., Ball, Lauren E., Denu, John M., Garcia, Benjamin A.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.07.2016; The American Society for Biochemistry and Molecular Biology
• Subjects: Boronic Acids - chemistry; Chromatography, Liquid - methods; Glycosylation; HeLa Cells; Humans; Isotope Labeling; Protein Processing, Post-Translational; Proteome - analysis; Proteome - chemistry; Proteomics - methods; Spectrometry, Mass, Electrospray Ionization - methods; Technological Innovation and Resources

Over the past decades, protein O-GlcNAcylation has been found to play a fundamental role in cell cycle control, metabolism, transcriptional regulation, and cellular signaling. Nevertheless, quantitative approaches to determine in vivo GlcNAc dynamics at a large-scale are still not readily available....