Simultaneous determination of aflatoxins, T-2 and HT-2 toxins, and fumonisins in cereal-derived products by QuEChERS extraction coupled with LC-MS/MS
A fast, easy, and cheap method for the simultaneous determination and quantification of aflatoxins (B1, B2, G1, G2), T-2 and HT-2 toxins, and fumonisins (B1, B2) in cereal-derived products was developed. This method involved a quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction cou...
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| Published in | Analytical and bioanalytical chemistry Vol. 409; no. 21; pp. 5143 - 5155 |
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| Main Authors | , , , , , , , |
| Format | Journal Article |
| Language | English |
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Berlin/Heidelberg
Springer Berlin Heidelberg
01.08.2017
Springer Springer Nature B.V |
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| Abstract | A fast, easy, and cheap method for the simultaneous determination and quantification of aflatoxins (B1, B2, G1, G2), T-2 and HT-2 toxins, and fumonisins (B1, B2) in cereal-derived products was developed. This method involved a quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction coupled with liquid chromatography-tandem mass spectrometry. The method validation was performed by analyzing samples spiked at four levels, and the recoveries ranged from 83.6 to 102.9%, whereas the maximum values of repeatability and within-laboratory reproducibility were 14.3 and 15.7% following the performance criteria set by the European legislation. The method was then applied for the analysis of 21 cereal-derived products purchased on the Italian market, which were correctly packaged and labeled as intended for human consumption. The co-occurrence of more than one mycotoxin in the analyzed samples could represent a risk for consumers, and the described method could be a valid alternative for their simultaneous detection in the framework of official control.
Graphical Abstract
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| AbstractList | A fast, easy, and cheap method for the simultaneous determination and quantification of aflatoxins (B1, B2, G1, G2), T-2 and HT-2 toxins, and fumonisins (B1, B2) in cereal-derived products was developed. This method involved a quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction coupled with liquid chromatography-tandem mass spectrometry. The method validation was performed by analyzing samples spiked at four levels, and the recoveries ranged from 83.6 to 102.9%, whereas the maximum values of repeatability and within-laboratory reproducibility were 14.3 and 15.7% following the performance criteria set by the European legislation. The method was then applied for the analysis of 21 cereal-derived products purchased on the Italian market, which were correctly packaged and labeled as intended for human consumption. The co-occurrence of more than one mycotoxin in the analyzed samples could represent a risk for consumers, and the described method could be a valid alternative for their simultaneous detection in the framework of official control. Graphical Abstract ᅟ. A fast, easy, and cheap method for the simultaneous determination and quantification of aflatoxins (B1, B2, G1, G2), T-2 and HT-2 toxins, and fumonisins (B1, B2) in cereal-derived products was developed. This method involved a quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction coupled with liquid chromatography-tandem mass spectrometry. The method validation was performed by analyzing samples spiked at four levels, and the recoveries ranged from 83.6 to 102.9%, whereas the maximum values of repeatability and within-laboratory reproducibility were 14.3 and 15.7% following the performance criteria set by the European legislation. The method was then applied for the analysis of 21 cereal-derived products purchased on the Italian market, which were correctly packaged and labeled as intended for human consumption. The co-occurrence of more than one mycotoxin in the analyzed samples could represent a risk for consumers, and the described method could be a valid alternative for their simultaneous detection in the framework of official control. Graphical Abstract ᅟ A fast, easy, and cheap method for the simultaneous determination and quantification of aflatoxins (B1, B2, G1, G2), T-2 and HT-2 toxins, and fumonisins (B1, B2) in cereal-derived products was developed. This method involved a quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction coupled with liquid chromatography-tandem mass spectrometry. The method validation was performed by analyzing samples spiked at four levels, and the recoveries ranged from 83.6 to 102.9%, whereas the maximum values of repeatability and within-laboratory reproducibility were 14.3 and 15.7% following the performance criteria set by the European legislation. The method was then applied for the analysis of 21 cereal-derived products purchased on the Italian market, which were correctly packaged and labeled as intended for human consumption. The co-occurrence of more than one mycotoxin in the analyzed samples could represent a risk for consumers, and the described method could be a valid alternative for their simultaneous detection in the framework of official control. |
| Audience | Academic |
| Author | Stramenga, Arianna Campana, Guido Colagrande, Maria Novella Visciano, Pierina De Colli, Lorenzo Schirone, Maria Scortichini, Giampiero Annunziata, Loredana |
| Author_xml | – sequence: 1 givenname: Loredana surname: Annunziata fullname: Annunziata, Loredana organization: Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise “G. Caporale” – sequence: 2 givenname: Arianna surname: Stramenga fullname: Stramenga, Arianna organization: Istituto Zooprofilattico Sperimentale dell’Umbria e delle Marche – sequence: 3 givenname: Pierina surname: Visciano fullname: Visciano, Pierina email: pvisciano@unite.it organization: Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo – sequence: 4 givenname: Maria surname: Schirone fullname: Schirone, Maria organization: Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo – sequence: 5 givenname: Lorenzo surname: De Colli fullname: De Colli, Lorenzo organization: Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise “G. Caporale” – sequence: 6 givenname: Maria Novella surname: Colagrande fullname: Colagrande, Maria Novella organization: Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise “G. Caporale” – sequence: 7 givenname: Guido surname: Campana fullname: Campana, Guido organization: Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise “G. Caporale” – sequence: 8 givenname: Giampiero surname: Scortichini fullname: Scortichini, Giampiero organization: Istituto Zooprofilattico Sperimentale dell’Umbria e delle Marche |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/28646297$$D View this record in MEDLINE/PubMed |
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| Keywords | Liquid chromatography-tandem mass spectrometry Cereal-derived products Method validation Mycotoxins |
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| Title | Simultaneous determination of aflatoxins, T-2 and HT-2 toxins, and fumonisins in cereal-derived products by QuEChERS extraction coupled with LC-MS/MS |
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