Efficient purification and metabolite analysis of radiotracers using high-performance liquid chromatography and on-line solid-phase extraction

• Published in: Journal of Chromatography A Vol. 1189; no. 1; pp. 323 - 331
• Main Authors: Chitneni, Satish K., Serdons, Kim, Evens, Nele, Fonge, Humphrey, Celen, Sofie, Deroose, Christophe M., Debyser, Zeger, Mortelmans, Luc, Verbruggen, Alfons M., Bormans, Guy M.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier B.V 02.05.2008; Amsterdam; New York: Elsevier; Elsevier
• Subjects: Animals; Biological and medical sciences; Chromatography, High Pressure Liquid - instrumentation; Chromatography, High Pressure Liquid - methods; Contrast media. Radiopharmaceuticals; high performance liquid chromatography; Humans; Medical sciences; Metabolite analysis; metabolites; Mice; Molecular Structure; Oasis ® HLB column; On-line solid-phase extraction; Pharmacology. Drug treatments; Positron-Emission Tomography; proteins; Radioisotopes - administration & dosage; Radioisotopes - blood; Radioisotopes - isolation & purification; Radiotracer; solid phase extraction; Solid Phase Extraction - methods; tracer techniques; urine; Metabolite analysis; Oasis ® HLB column; Radiotracer; On-line solid-phase extraction; Solid phase extraction; Biological fluid; Embryo; Chemical analysis; Metabolite; HPLC chromatography; Kidney; Blood; Chemical enrichment; Blood plasma; Sample preparation; Scintigraphic agent; Radioisotopic product; Trace analysis; Human; Urine; Purification; Oasis® HLB column; Coupled method; Rodentia; Vertebrata; Reversed phase chromatography; Mammalia; Ultraviolet detector; Cell line; Mouse; Animal

This study describes an efficient method using on-line solid-phase extraction (SPE) (Oasis ® HLB) for preparative HPLC purification of short-lived radiotracers for positron emission tomography (PET) and for HPLC analysis of radiotracers and their metabolites in cell homogenates, plasma and urine sam...