Identification of repertoires of surface antigens on leukemias using an antibody microarray

• Published in: Proteomics (Weinheim) Vol. 3; no. 11; pp. 2147 - 2154
• Main Authors: Belov, Larissa, Huang, Pauline, Barber, Nicole, Mulligan, Stephen P., Christopherson, Richard I.
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 01.11.2003; WILEY‐VCH Verlag
• Subjects: Antibodies - immunology; Antibody; antibody microarrays; Antigens, Surface - immunology; Antigens, Surface - metabolism; Cluster of differentiation antigens; Collodion - chemistry; HL-60 Cells; Humans; Leukemia; Leukemia - diagnosis; Leukemia - drug therapy; Leukemia - immunology; Leukocytes - immunology; Lymphoma; Lymphoma - diagnosis; Lymphoma - drug therapy; Lymphoma - immunology; Microarray; Tretinoin - pharmacology

We have previously described a microarray of cluster of differentiation (CD) antibodies that enables concurrent determination of more than 60 CD antigens on leukocytes. This procedure does not require protein purification or labeling, or a secondary detection system. Whole cells are captured by a mi...