An Optimized Two‐Photon Fluorescent Probe for Biological Sensing and Imaging of Catechol‐O‐Methyltransferase

• Published in: Chemistry : a European journal Vol. 23; no. 45; pp. 10800 - 10807
• Main Authors: Wang, Ping, Xia, Yang‐Liu, Zou, Li‐Wei, Qian, Xing‐Kai, Dou, Tong‐Yi, Jin, Qiang, Li, Shi‐Yang, Yu, Yang, Wang, Dan‐Dan, Luo, Qun, Ge, Guang‐Bo, Yang, Ling
• Format: Journal Article
• Language: English
• Published: WEINHEIM Wiley 10.08.2017; Wiley Subscription Services, Inc
• Subjects: Animals; Binding Sites; bio-imaging; Biological activity; Biological properties; Biological samples; biosensing; Brain - metabolism; Catechol; Catechol O-methyltransferase; Catechol O-Methyltransferase - chemistry; Catechol O-Methyltransferase - metabolism; Cell Line, Tumor; Cell permeability; Cells (biology); Chemistry; Chemistry, Multidisciplinary; coumarins; Coumarins - chemical synthesis; Coumarins - chemistry; Coumarins - metabolism; Cytotoxicity; Enzymes; Fluorescence; Fluorescent Dyes - chemical synthesis; Fluorescent Dyes - chemistry; Fluorescent Dyes - metabolism; Fluorescent indicators; Humans; Image resolution; Imaging; Kinetics; Methylation; Methyltransferase; Microscopy, Fluorescence, Multiphoton; Molecular Docking Simulation; Photons; Physical Sciences; Rats; Science & Technology; Selectivity; Spectrometry, Fluorescence; Toxicity; METHYLATION; DESIGN; coumarins; SCHIZOPHRENIA; DISORDERS; POLYMORPHISM; COMT; bio-imaging; catechol-o-methyltransferase; fluorescence; biosensing; NITRIC-OXIDE; INHIBITORS; PARKINSONS-DISEASE; LIVING CELLS

A practical two‐photon fluorescent probe was developed for highly sensitive and selective sensing of the activities of catechol‐O‐methyltransferase (COMT) in complex biological samples. To this end, a series of 3‐substituted 7,8‐dihydroxycoumarins were designed and synthesized. Among them, 3‐BTD dis...