Circ‐AKT3 inhibits the accumulation of extracellular matrix of mesangial cells in diabetic nephropathy via modulating miR‐296‐3p/E‐cadherin signals

• Published in: Journal of cellular and molecular medicine Vol. 24; no. 15; pp. 8779 - 8788
• Main Authors: Tang, Bo, Li, Weiliang, Ji, Ting‐Ting, Li, Xiao‐Ying, Qu, Xiaolei, Feng, Linhong, Bai, Shoujun
• Format: Journal Article
• Language: English
• Published: England John Wiley & Sons, Inc 01.08.2020; John Wiley and Sons Inc
• Subjects: Animal models; Animals; Antibodies; Apoptosis; Apoptosis - genetics; Bioinformatics; Cadherins - genetics; circ‐AKT3; Collagen (type I); Collagen (type IV); Diabetes; Diabetes mellitus; Diabetic Nephropathies - etiology; Diabetic Nephropathies - metabolism; Diabetic Nephropathies - pathology; Diabetic nephropathy; Disease; Disease Progression; Disease Susceptibility; Extracellular matrix; Extracellular Matrix - metabolism; E‐cadherin; Fibronectin; Fibrosis; Flow cytometry; Gene expression; Gene Expression Regulation; Genes, Reporter; Glucose; Kidneys; Laboratory animals; Matrix protein; Medical research; Membranes; Mesangial cells; Mesangial Cells - metabolism; Mesangial Cells - pathology; Metabolism; Mice; MicroRNAs; MicroRNAs - genetics; miR‐296‐3p; Nephropathy; Original; Pathogenesis; Proteins; Proto-Oncogene Proteins c-akt - genetics; RNA Interference; RNA, Circular; Signal Transduction; Statistical analysis; Studies; miR-296-3p; E-cadherin; circ-AKT3; diabetic nephropathy
• DOI: 10.1111/jcmm.15513
• ISSN: 1582-1838; 1582-4934

Diabetic nephropathy is a leading cause of end‐stage renal disease globally. The vital role of circular RNAs (circRNAs) has been reported in diabetic nephropathy progression, but the molecular mechanism linking diabetic nephropathy to circRNAs remains elusive. In this study, we investigated the significant function of circ‐AKT3/miR‐296‐3p/E‐cadherin regulatory network on the extracellular matrix accumulation in mesangial cells in diabetic nephropathy. The expression of circ‐AKT3 and fibrosis‐associated proteins, including fibronectin, collagen type I and collagen type IV, was assessed via RT‐PCR and Western blot analysis in diabetic nephropathy animal model and mouse mesangial SV40‐MES13 cells. Luciferase reporter assays were used to investigate interactions among E‐cadherin, circ‐AKT3 and miR‐296‐3p in mouse mesangial SV40‐MES13 cells. Cell apoptosis was evaluated via flow cytometry. The level of circ‐AKT3 was significantly lower in diabetic nephropathy mice model group and mouse mesangial SV40‐MES13 cells treated with high‐concentration (25 mmol/L) glucose. In addition, circ‐AKT3 overexpression inhibited the level of fibrosis‐associated protein, such as fibronectin, collagen type I and collagen type IV. Circ‐AKT3 overexpression also inhibited the apoptosis of mouse mesangial SV40‐MES13 cells treated with high glucose. Luciferase reporter assay and bioinformatics tools identified that circ‐AKT3 could act as a sponge of miR‐296‐3p and E‐cadherin was the miR‐296‐3p direct target. Moreover, circ‐AKT3/miR‐296‐3p/E‐cadherin modulated the extracellular matrix of mouse mesangial cells in high‐concentration (25 mmol/L) glucose, inhibiting the synthesis of related extracellular matrix protein. In conclusion, circ‐AKT3 inhibited the extracellular matrix accumulation in diabetic nephropathy mesangial cells through modulating miR‐296‐3p/E‐cadherin signals, which might offer novel potential opportunities for clinical diagnosis targets and therapeutic biomarkers for diabetic nephropathy.