Binding and screening by cations and the effect on exogenous NAD(P)H oxidation in Neurospora crassa mitochondria

• Published in: European journal of biochemistry Vol. 123; no. 1; pp. 81 - 88
• Main Authors: Moller, I.M, Schwitzguebel, J.P, Palmer, J.M
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.03.1982
• Subjects: Aminacrine; Binding Sites; cations; Cations - pharmacology; Cell Membrane - enzymology; Chelating Agents - pharmacology; fungi; Hydrogen-Ion Concentration; Lanthanum - pharmacology; Membrane Potentials; membranes; mitochondria; Mitochondria - enzymology; NADH, NADPH Oxidoreductases - metabolism; NADPH; Neurospora - enzymology; Neurospora crassa; Neurospora crassa - enzymology; oxidation; Species Specificity

1 The uncoupled oxidation of exogenous NADH by mitochondria from Neurospora crassu has a pH optimum at 7.0. In the presence of EDTA (1 mM) the optimum is at pH 6.5; maximal inhibition (65%) occurs at pH 7.2. This is comparable to the results with higher plant mitochondria. 2 The corresponding pH opt...