M Cell DNA Vaccination for CTL Immunity to HIV

• Published in: The Journal of immunology (1950) Vol. 171; no. 9; pp. 4717 - 4725
• Main Authors: Wang, Xinhai, Hone, David M, Haddad, Asmahan, Shata, Mohamed T, Pascual, David W
• Format: Journal Article
• Language: English
• Published: United States Am Assoc Immnol 01.11.2003
• Subjects: Administration, Intranasal; AIDS Vaccines - administration & dosage; AIDS Vaccines - genetics; AIDS Vaccines - immunology; Animals; Capsid Proteins - administration & dosage; Capsid Proteins - genetics; Capsid Proteins - immunology; Cytotoxicity, Immunologic - genetics; DNA, Viral - administration & dosage; DNA, Viral - immunology; Dose-Response Relationship, Immunologic; Female; HIV Infections - immunology; HIV Infections - prevention & control; HIV-1 - genetics; HIV-1 - immunology; Immunity, Mucosal - genetics; Immunization Schedule; Interferon-gamma - physiology; Lymphocyte Activation - genetics; Male; Membrane Glycoproteins - physiology; Mice; Mice, Inbred BALB C; Mice, Knockout; Perforin; Peyer's Patches - cytology; Peyer's Patches - immunology; Pore Forming Cytotoxic Proteins; T-Lymphocytes, Cytotoxic - immunology; Vaccines, DNA - administration & dosage; Vaccines, DNA - immunology; Vaccinia virus - genetics; Vaccinia virus - immunology; Viral Envelope Proteins - biosynthesis; Viral Envelope Proteins - genetics; Viral Envelope Proteins - immunology

To facilitate invasion, reovirus has evolved to attach to M cells, a specialized epithelium residing within the follicle-associated epithelium that covers mucosal inductive tissues. Thus, we questioned adapting reovirus protein sigma1 to ferry DNA vaccines to the mucosa to immunize against HIV. Thre...