Identifying eIF4E-binding protein translationally-controlled transcripts reveals links to mRNAs bound by specific PUF proteins

• Published in: Nucleic acids research Vol. 38; no. 22; pp. 8039 - 8050
• Main Authors: Cridge, Andrew G, Castelli, Lydia M, Smirnova, Julia B, Selley, Julian N, Rowe, William, Hubbard, Simon J, McCarthy, John E G, Ashe, Mark P, Grant, Christopher M, Pavitt, Graham D
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.12.2010
• Subjects: Amino Acids - metabolism; Cyclins - genetics; Eukaryotic Initiation Factors - genetics; Eukaryotic Initiation Factors - physiology; Gene Deletion; Gene Expression Regulation, Fungal; Gene Regulation, Chromatin and Epigenetics; Nitrogen - metabolism; Oligonucleotide Array Sequence Analysis; Protein Biosynthesis; RNA, Messenger - metabolism; RNA-Binding Proteins - metabolism; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - growth & development; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae Proteins - genetics; Saccharomyces cerevisiae Proteins - physiology; Transcription Factors - genetics; Transcription Factors - physiology; Transcription, Genetic

eIF4E-binding proteins (4E-BPs) regulate translation of mRNAs in eukaryotes. However the extent to which specific mRNA targets are regulated by 4E-BPs remains unknown. We performed translational profiling by microarray analysis of polysome and monosome associated mRNAs in wild-type and mutant cells...