Chemoattractant-Stimulated Rac Activation in Wild-Type and Rac2-Deficient Murine Neutrophils: Preferential Activation of Rac2 and Rac2 Gene Dosage Effect on Neutrophil Functions

• Published in: The Journal of immunology (1950) Vol. 169; no. 9; pp. 5043 - 5051
• Main Authors: Li, Shijun, Yamauchi, Akira, Marchal, Christophe C, Molitoris, Jason K, Quilliam, Lawrence A, Dinauer, Mary C
• Format: Journal Article
• Language: English
• Published: United States Am Assoc Immnol 01.11.2002
• Subjects: Actins - metabolism; Animals; Chemotaxis, Leukocyte - genetics; Chemotaxis, Leukocyte - immunology; Enzyme Inhibitors - pharmacology; Female; Gene Dosage; Genetic Carrier Screening; Humans; Male; Mice; Mice, Inbred C57BL; Mice, Knockout; N-Formylmethionine Leucyl-Phenylalanine - pharmacology; NADPH Oxidases - deficiency; NADPH Oxidases - genetics; Neutrophil Activation - genetics; Neutrophil Activation - immunology; Neutrophils - enzymology; Neutrophils - immunology; Neutrophils - metabolism; Phosphoinositide-3 Kinase Inhibitors; Protein Isoforms - biosynthesis; Protein Isoforms - deficiency; Protein Isoforms - genetics; Protein Isoforms - metabolism; rac GTP-Binding Proteins - biosynthesis; rac GTP-Binding Proteins - deficiency; rac GTP-Binding Proteins - genetics; rac GTP-Binding Proteins - metabolism; rac1 GTP-Binding Protein - biosynthesis; rac1 GTP-Binding Protein - metabolism; RAC2 GTP-Binding Protein; src-Family Kinases - antagonists & inhibitors

The hemopoietic-specific Rho family GTPase Rac2 shares 92% amino acid identity with ubiquitously expressed Rac1. Neutrophils from rac2(-/-) mice have multiple defects, including chemoattractant-stimulated NADPH oxidase activity and chemotaxis, which may result from an overall reduction in cellular R...