Construction of an Integration Vector with a Chimeric Signal Peptide for the Expression of Monoclonal Antibodies in Mammalian Cells

• Published in: Current Issues in Molecular Biology Vol. 46; no. 12; pp. 14464 - 14475
• Main Authors: Nesmeyanova, Valentina S., Shanshin, Daniil V., Murashkin, Denis E., Shcherbakov, Dmitriy N.
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.12.2024; MDPI
• Subjects: Analysis; artificial signal sequence; Cells; CHO-K1; Genetic engineering; integrative vectors; Monoclonal antibodies; Peptides; recombinant antibodies; Recombinant proteins; United States; Massachusetts; China; integrative vectors; recombinant antibodies; artificial signal sequence; CHO-K1

Antibodies are complex protein structures, and producing them using eukaryotic expression systems presents significant challenges. One frequently overlooked aspect of expression vectors is the nucleotide sequence encoding the signal peptide, which plays a pivotal role in facilitating the secretion o...