Functional genomic screens with death rate analyses reveal mechanisms of drug action

• Published in: Nature chemical biology Vol. 20; no. 11; pp. 1443 - 1452
• Main Authors: Honeywell, Megan E., Isidor, Marie S., Harper, Nicholas W., Fontana, Rachel E., Birdsall, Gavin A., Cruz-Gordillo, Peter, Porto, Sydney A., Jerome, Madison, Fraser, Cameron S., Sarosiek, Kristopher A., Guertin, David A., Spinelli, Jessica B., Lee, Michael J.
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.11.2024; Nature Publishing Group
• Subjects: 631/67/1059; 631/80/82; 631/92/360; 631/92/609; 631/92/93; Antineoplastic Agents - pharmacology; Apoptosis; Apoptosis - drug effects; Apoptosis - genetics; Biochemical Engineering; Biochemistry; Bioorganic Chemistry; Cell Biology; Cell death; Cell Death - drug effects; Cell Death - genetics; Cell Line, Tumor; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Damage detection; Deoxyribonucleic acid; DNA; DNA damage; DNA Damage - drug effects; DNA fingerprinting; Effectiveness; Genes; Genetic analysis; Genetic diversity; Genomics - methods; Humans; Lethality; Mortality; p53 Protein; Tumor Suppressor Protein p53 - genetics; Tumor Suppressor Protein p53 - metabolism

A common approach for understanding how drugs induce their therapeutic effects is to identify the genetic determinants of drug sensitivity. Because ‘chemo-genetic profiles’ are performed in a pooled format, inference of gene function is subject to several confounding influences related to variation...