Transient receptor potential vanilloid 4 inhibits mouse colonic motility by activating NO-dependent enteric neurotransmission

• Published in: Journal of molecular medicine (Berlin, Germany) Vol. 93; no. 12; pp. 1297 - 1309
• Main Authors: Fichna, J., Poole, D. P., Veldhuis, N., MacEachern, S. J., Saur, D, Zakrzewski, P. K., Cygankiewicz, A. I., Mokrowiecka, A., Małecka-Panas, E., Krajewska, W. M., Liedtke, W., Steinhoff, M. S., Timmermans, J-P., Bunnett, N. W., Sharkey, K. A., Storr, M. A.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.12.2015; Springer Nature B.V
• Subjects: Adult; Aged; Aged, 80 and over; Animals; Biomedical and Life Sciences; Biomedicine; Case-Control Studies; Colon - drug effects; Colon - physiology; Colon - physiopathology; Disease Models, Animal; Female; Gastrointestinal Motility - drug effects; Gastrointestinal Motility - genetics; Gene Expression; Guanylate Cyclase - metabolism; Human Genetics; Humans; Internal Medicine; Irritable Bowel Syndrome - drug therapy; Irritable Bowel Syndrome - genetics; Irritable Bowel Syndrome - metabolism; Irritable Bowel Syndrome - physiopathology; Leucine - analogs & derivatives; Leucine - pharmacology; Male; Mice; Mice, Knockout; Middle Aged; Models, Biological; Molecular Medicine; Muscle Contraction - drug effects; Muscle, Smooth - drug effects; Muscle, Smooth - metabolism; Myenteric Plexus - metabolism; Nitric Oxide - metabolism; Nitric Oxide Synthase - metabolism; Original Article; Sulfonamides - pharmacology; Synaptic Transmission - genetics; TRPV Cation Channels - antagonists & inhibitors; TRPV Cation Channels - genetics; TRPV Cation Channels - metabolism; Myenteric plexus; Nitric oxide synthase type 1; Irritable bowel syndrome

Recent studies implicate TRPV4 receptors in visceral pain signaling and intestinal inflammation. Our aim was to evaluate the role of TRPV4 in the control of gastrointestinal (GI) motility and to establish the underlying mechanisms. We used immunohistochemistry and PCR to study TRPV4 expression in th...