A new method to estimate respiration rate of biological material based on the reduction of tetrazolium violet

• Published in: Journal of experimental marine biology and ecology Vol. 251; no. 2; pp. 239 - 263
• Main Author: BAMSTEDT, U
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 30.08.2000; Elsevier Science
• Subjects: Animal, plant and microbial ecology; Biological and medical sciences; Enzymatic reduction; Fundamental and applied biological sciences. Psychology; General aspects. Techniques; Marine; Methods and techniques (sampling, tagging, trapping, modelling...); Respiration; Sweden; Tetrazolium violet; Enzymatic reduction; Respiration; Tetrazolium violet; Tetrazolium test; Chemical reagent; Experimental plan; Environmental factor; Mysidacea; Crustacea; Marine environment; Aquatic environment; Vertebrata; Analysis method; Enzymatic activity; Arthropoda; Pisces; Technique; Invertebrata

The traditional method for measuring the activity of the electron-transport system (ETS) gives an estimate of the potential respiration rate, since it measures the rate under saturated substrate conditions ( V max). Accumulated literature data indicate that this does not relate closely to the actual...