3D bioengineered neural tissue generated from patient-derived iPSCs mimics time-dependent phenotypes and transcriptional features of Alzheimer’s disease

• Published in: Molecular psychiatry Vol. 28; no. 12; pp. 5390 - 5401
• Main Authors: Lomoio, Selene, Pandey, Ravi S., Rouleau, Nicolas, Menicacci, Beatrice, Kim, WonHee, Cantley, William L., Haydon, Philip G., Bennett, David A., Young-Pearse, Tracy L., Carter, Gregory W., Kaplan, David L., Tesco, Giuseppina
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.12.2023; Nature Publishing Group
• Subjects: 13/100; 13/106; 13/107; 14/19; 38; 45; 631/378; 631/80; 82/51; 9/30; 96/106; 96/63; Aging; Alzheimer Disease - genetics; Alzheimer Disease - metabolism; Alzheimer's disease; Amyloid beta-Peptides - metabolism; Amyloid beta-Protein Precursor - genetics; Amyloid beta-Protein Precursor - metabolism; Behavioral Sciences; Bioengineering - methods; Biological Psychology; Brain; Brain - metabolism; Cell culture; Cell Differentiation; Cloning; Dementia; Disease; Excitability; Gene expression; Glial cells; Humans; Hydrogels; Induced Pluripotent Stem Cells - metabolism; Inhibitory postsynaptic potentials; Medicine; Medicine & Public Health; Mutation; Neurodegenerative diseases; Neuroglia - metabolism; Neuronal-glial interactions; Neurons - metabolism; Neurosciences; Patients; Pharmacotherapy; Phenotype; Phenotypes; Proteins; Psychiatry; Stem cells; Transcriptome - genetics; Transcriptomics; β-Amyloid

Several iPSC-derived three-dimensional (3D) cultures have been generated to model Alzheimer’s disease (AD). While some AD-related phenotypes have been identified across these cultures, none of them could recapitulate multiple AD-related hallmarks in one model. To date, the transcriptomic features of...