Validation tests for cryo-EM maps using an independent particle set

• Published in: Journal of structural biology. X Vol. 4; p. 100032
• Main Authors: Ortiz, Sebastian, Stanisic, Luka, Rodriguez, Boris A, Rampp, Markus, Hummer, Gerhard, Cossio, Pilar
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.01.2020; Elsevier
• Subjects: 3D refinement; BioEM; Cryo-EM; Independent; Raw data; Reconstruction; Validation; Validation; Reconstruction; Independent; BioEM; Cryo-EM; 3D refinement; Raw data

[Display omitted] •Use of an independent particle set to validate 3D density maps from cryo-EM.•The posterior probability of the map should increase as a function of the iteration and low-pass frequency cutoff.•The similarity between the distributions of the posterior probabilities is an indicator o...