Rapid re-identification of human samples using portable DNA sequencing
DNA re-identification is used for a broad suite of applications, ranging from cell line authentication to forensics. However, current re-identification schemes suffer from high latency and limited access. Here, we describe a rapid, inexpensive, and portable strategy to robustly re-identify human DNA...
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| Published in | eLife Vol. 6 |
|---|---|
| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
England
eLife Sciences Publications Ltd
28.11.2017
eLife Sciences Publications, Ltd |
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| Online Access | Get full text |
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| Abstract | DNA re-identification is used for a broad suite of applications, ranging from cell line authentication to forensics. However, current re-identification schemes suffer from high latency and limited access. Here, we describe a rapid, inexpensive, and portable strategy to robustly re-identify human DNA called 'MinION sketching'. MinION sketching requires as few as 3 min of sequencing and 60-300 random SNPs to re-identify a sample enabling near real-time applications of DNA re-identification. Our method capitalizes on the rapidly growing availability of genomic reference data for cell lines, tissues in biobanks, and individuals. This empowers the application of MinION sketching in research and clinical settings for periodic cell line and tissue authentication. Importantly, our method enables considerably faster and more robust cell line authentication relative to current practices and could help to minimize the amount of irreproducible research caused by mix-ups and contamination in human cell and tissue cultures. |
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| AbstractList | DNA re-identification is used for a broad suite of applications, ranging from cell line authentication to forensics. However, current re-identification schemes suffer from high latency and limited access. Here, we describe a rapid, inexpensive, and portable strategy to robustly re-identify human DNA called 'MinION sketching'. MinION sketching requires as few as 3 min of sequencing and 60-300 random SNPs to re-identify a sample enabling near real-time applications of DNA re-identification. Our method capitalizes on the rapidly growing availability of genomic reference data for cell lines, tissues in biobanks, and individuals. This empowers the application of MinION sketching in research and clinical settings for periodic cell line and tissue authentication. Importantly, our method enables considerably faster and more robust cell line authentication relative to current practices and could help to minimize the amount of irreproducible research caused by mix-ups and contamination in human cell and tissue cultures. DNA re-identification is used for a broad suite of applications, ranging from cell line authentication to forensics. However, current re-identification schemes suffer from high latency and limited access. Here, we describe a rapid, inexpensive, and portable strategy to robustly re-identify human DNA called 'MinION sketching'. MinION sketching requires as few as 3 min of sequencing and 60-300 random SNPs to re-identify a sample enabling near real-time applications of DNA re-identification. Our method capitalizes on the rapidly growing availability of genomic reference data for cell lines, tissues in biobanks, and individuals. This empowers the application of MinION sketching in research and clinical settings for periodic cell line and tissue authentication. Importantly, our method enables considerably faster and more robust cell line authentication relative to current practices and could help to minimize the amount of irreproducible research caused by mix-ups and contamination in human cell and tissue cultures. The human genome represents the complete set of genetic information needed to make a person. DNA sequencing technologies used to study genomes have become much faster, cheaper and more accessible over recent years. This has enabled them to be used more regularly in various fields like precision medicine, in research laboratories and forensics. Even so, there are still fields where optimization is critical. Reproducibility is an important issue in biomedical research; one group of scientists working with human cells may report results that other scientists cannot reproduce. Sometimes this is because the original work was done in the wrong type of cells by mistake. Human cells used in biomedical research are very hard to discriminate from each other using microscopes; however, DNA analysis can be used to ensure the origin of the cells. The MinION device, a USB compatible handheld DNA sequencer, has become available in the last few years. Its size, speed and portability could enable many new uses for DNA sequencing. Technology like this could be used to confirm which cells the scientists are working with before they publish their results. Yet, currently DNA readings from the MinION are not accurate enough to be used to reliably confirm the identity of human cells used in research. Zaaijer et al. have now developed an approach that can accurately identify human cells using the MinION device. The approach involves “DNA re-identification”, which works by comparing an unknown DNA sample to a collection of known DNA profiles. Using their new method, Zaaijer et al. report that, with three minutes of DNA sequencing, they can correctly identify a DNA sample, with 99.9% confidence. This is a high enough level of accuracy for the system to tell the difference between one person and another, using only their DNA. This new technology is much faster than current rapid DNA sequencing approaches. Previously, processing DNA samples could take hours or even days and was not particularly portable. The new technology has many applications from finding criminals to diagnosing illnesses and tracking epidemics. It is also an affordable way for laboratories to confirm the identity of cells they are working with. This has the potential to save billions in research funding each year and speed up scientific progress. |
| Author | Gordon, Assaf Piccone, Robert Erlich, Yaniv Groen, Simon Cornelis Speyer, Daniel Zaaijer, Sophie |
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| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/29182147$$D View this record in MEDLINE/PubMed |
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| Cites_doi | 10.1093/bioinformatics/btu555 10.18632/oncotarget.16110 10.12688/f1000research.7201.1 10.1002/ijc.25242 10.7554/eLife.14258 10.1186/s13059-015-0677-2 10.1126/science.1229566 10.1126/science.1181498 10.1038/nrg1455 10.1038/nature11003 10.1126/science.1095019 10.1038/nature14397 10.1371/journal.pbio.1002476 10.1073/pnas.121616198 10.1038/nrc2852 10.1002/elps.200500671 10.1002/ijc.27675 10.1038/nbt.2408 10.1038/217750a0 10.1126/science.1122655 10.1038/nrg2952 10.18632/oncotarget.11125 |
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| Copyright | 2017, Zaaijer et al. This work is licensed under the Creative Commons Attribution License ( https://creativecommons.org/licenses/by/3.0/ ) (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. 2017, Zaaijer et al 2017 Zaaijer et al |
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| DOI | 10.7554/eLife.27798 |
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| Keywords | nanopore sequencing forensics cell biology evolutionary biology cell line authentication re-identification genomics DNA fingerprinting human |
| Language | English |
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| References | Masters (bib24) 2001; 98 Reid (bib28) 2013 Capes-Davis (bib8) 2010; 127 Erlich (bib14) 2017 Green (bib16) 2013; 4472 Zaaijer (bib32) 2016; 5 Loman (bib23) 2014; 30 Gymrek (bib17) 2013; 339 Ip (bib18) 2015; 4 Kayser (bib20) 2011; 12 Li (bib21) 2013 Smith (bib30) 2012; 30 Capes-Davis (bib7) 2016 Sanchez (bib29) 2006; 27 Lin (bib22) 2004; 305 Bieber (bib6) 2006; 312 Erlich (bib13) 2015 Otto (bib26) 2017; 8 Castro (bib10) 2013; 132 Quick (bib27) 2015; 16 AMS (bib3) 2015 El-Hoss (bib12) 2016; 7 Barretina (bib5) 2012; 483 Alston-Roberts (bib2) 2010; 10 ATCC (bib4) 2011 Almeida (bib1) 2016; 14 Capes-Davis (bib9) 2013; 2519 Yu (bib31) 2015; 520 NIH (bib25) 2016 Gartler (bib15) 1968; 217 Jobling (bib19) 2004; 5 Drmanac (bib11) 2010; 327 |
| References_xml | – volume: 30 start-page: 3399 year: 2014 ident: bib23 article-title: Poretools: a toolkit for analyzing nanopore sequence data publication-title: Bioinformatics doi: 10.1093/bioinformatics/btu555 contributor: fullname: Loman – year: 2016 ident: bib7 article-title: Letter to joe biden, moonshot initiative contributor: fullname: Capes-Davis – volume: 8 start-page: 34310 year: 2017 ident: bib26 article-title: Robust in-silico identification of cancer cell lines based on next generation sequencing publication-title: Oncotarget doi: 10.18632/oncotarget.16110 contributor: fullname: Otto – volume: 4 year: 2015 ident: bib18 article-title: MinION analysis and reference consortium: phase 1 data release and analysis publication-title: F1000Research doi: 10.12688/f1000research.7201.1 contributor: fullname: Ip – volume-title: GitHub year: 2017 ident: bib14 article-title: personal-identification-pipeline contributor: fullname: Erlich – volume-title: Preprint year: 2013 ident: bib21 article-title: Aligning sequence reads, clone sequences and assembly contigs with BWA-MEM contributor: fullname: Li – volume: 127 start-page: 1 year: 2010 ident: bib8 article-title: Check your cultures! a list of cross-contaminated or misidentified cell lines publication-title: International Journal of Cancer doi: 10.1002/ijc.25242 contributor: fullname: Capes-Davis – volume: 5 start-page: e14258 year: 2016 ident: bib32 article-title: Using mobile sequencers in an academic classroom publication-title: eLife doi: 10.7554/eLife.14258 contributor: fullname: Zaaijer – volume: 16 start-page: 1 year: 2015 ident: bib27 article-title: Rapid draft sequencing and real-time nanopore sequencing in a hospital outbreak of Salmonella publication-title: Genome Biology doi: 10.1186/s13059-015-0677-2 contributor: fullname: Quick – volume-title: Presented at the 65th Annual Meeting of the American Society of Human Genetics year: 2015 ident: bib13 contributor: fullname: Erlich – volume: 339 start-page: 321 year: 2013 ident: bib17 article-title: Identifying personal genomes by surname inference publication-title: Science doi: 10.1126/science.1229566 contributor: fullname: Gymrek – volume: 327 start-page: 78 year: 2010 ident: bib11 article-title: Human genome sequencing using unchained base reads on self-assembling DNA nanoarrays publication-title: Science doi: 10.1126/science.1181498 contributor: fullname: Drmanac – volume: 5 start-page: 739 year: 2004 ident: bib19 article-title: Encoded evidence: DNA in forensic analysis publication-title: Nature Reviews Genetics doi: 10.1038/nrg1455 contributor: fullname: Jobling – year: 2015 ident: bib3 article-title: Reproducibility and reliability of biomedical research: improving research practice contributor: fullname: AMS – volume: 483 start-page: 603 year: 2012 ident: bib5 article-title: The cancer cell line encyclopedia enables predictive modelling of anticancer drug sensitivity publication-title: Nature doi: 10.1038/nature11003 contributor: fullname: Barretina – year: 2016 ident: bib25 article-title: Enhanced reproducibility through rigor and transparency (effective Jan. 25, 2016) contributor: fullname: NIH – volume: 4472 start-page: 1 year: 2013 ident: bib16 article-title: American College of medical genetics and genomics acmg recommendations for reporting of incidental findings in clinical exome and genome sequencing contributor: fullname: Green – volume: 305 start-page: 1095019 year: 2004 ident: bib22 article-title: Genomic research and human subject privacy publication-title: Science doi: 10.1126/science.1095019 contributor: fullname: Lin – volume: 520 start-page: 307 year: 2015 ident: bib31 article-title: A resource for cell line authentication, annotation and quality control publication-title: Nature doi: 10.1038/nature14397 contributor: fullname: Yu – volume: 14 start-page: e1002476 year: 2016 ident: bib1 article-title: Standards for cell line authentication and beyond publication-title: PLoS Biology doi: 10.1371/journal.pbio.1002476 contributor: fullname: Almeida – volume: 98 start-page: 8012 year: 2001 ident: bib24 article-title: Short tandem repeat profiling provides an international reference standard for human cell lines publication-title: PNAS doi: 10.1073/pnas.121616198 contributor: fullname: Masters – volume: 2519 start-page: 2510 year: 2013 ident: bib9 article-title: Match criteria for human cell line authentication:where do we draw the line? contributor: fullname: Capes-Davis – volume: 10 start-page: 441 year: 2010 ident: bib2 article-title: Cell line misidentification: the beginning of the end publication-title: Nature Reviews. Cancer doi: 10.1038/nrc2852 contributor: fullname: Alston-Roberts – year: 2011 ident: bib4 article-title: Authentication of human cell lines: Standardization of STR profiling contributor: fullname: ATCC – volume: 27 start-page: 1713 year: 2006 ident: bib29 article-title: A multiplex assay with 52 single nucleotide polymorphisms for human identification publication-title: Electrophoresis doi: 10.1002/elps.200500671 contributor: fullname: Sanchez – volume: 132 start-page: 308 year: 2013 ident: bib10 article-title: High-throughput SNP-based authentication of human cell lines publication-title: International Journal of Cancer doi: 10.1002/ijc.27675 contributor: fullname: Castro – volume: 30 start-page: 1047 year: 2012 ident: bib30 article-title: DNA goes to court publication-title: Nature Biotechnology doi: 10.1038/nbt.2408 contributor: fullname: Smith – volume: 217 start-page: 750 year: 1968 ident: bib15 article-title: Apparent hela cell contamination of human heteroploid cell lines publication-title: Nature doi: 10.1038/217750a0 contributor: fullname: Gartler – volume: 312 start-page: 1315 year: 2006 ident: bib6 article-title: Finding criminals through dna of their relatives publication-title: Science doi: 10.1126/science.1122655 contributor: fullname: Bieber – volume: 12 start-page: 179 year: 2011 ident: bib20 article-title: Improving human forensics through advances in genetics, genomics and molecular biology publication-title: Nature Reviews Genetics doi: 10.1038/nrg2952 contributor: fullname: Kayser – volume-title: Assay Guidance Manual year: 2013 ident: bib28 contributor: fullname: Reid – volume: 7 start-page: 60475 year: 2016 ident: bib12 article-title: A single nucleotide polymorphism genotyping platform for the authentication of patient derived xenografts publication-title: Oncotarget doi: 10.18632/oncotarget.11125 contributor: fullname: El-Hoss |
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| Snippet | DNA re-identification is used for a broad suite of applications, ranging from cell line authentication to forensics. However, current re-identification schemes... |
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| SubjectTerms | Cancer Cell Biology cell line authentication Cell lines Computational Biology - methods Computer science Contamination Data processing Deoxyribonucleic acid DNA DNA - chemistry DNA - genetics DNA fingerprinting DNA sequencing Forensic science Forensic sciences forensics Genetic testing Genetics and Genomics Genomes Genotyping Techniques - methods Humans Identification Laboratories Laboratory equipment Latency nanopore sequencing Polymorphism, Single Nucleotide Probability re-identification Sequence Analysis, DNA - methods Single-nucleotide polymorphism Time Factors Tools and Resources |
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| Title | Rapid re-identification of human samples using portable DNA sequencing |
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