The dynamic interplay of host and viral enzymes in type III CRISPR-mediated cyclic nucleotide signalling

• Published in: eLife Vol. 9
• Main Authors: Athukoralage, Januka S, Graham, Shirley, Rouillon, Christophe, Grüschow, Sabine, Czekster, Clarissa M, White, Malcolm F
• Format: Journal Article
• Language: English
• Published: England eLife Sciences Publications Ltd 27.04.2020; eLife Sciences Publications, Ltd
• Subjects: Antiviral agents; Biochemistry and Chemical Biology; Chromatography; CRISPR; CRISPR-Cas Systems; cyclic oligoadenylate; Enzymes; Escherichia coli - enzymology; Escherichia coli - genetics; Host Microbial Interactions; Immune system; Nuclease; Nucleotides, Cyclic - metabolism; Research Advance; Ribonuclease; Ribonucleic acid; ring nuclease; RNA; Second messengers; Signal Transduction; Sulfolobus solfataricus; Sulfolobus solfataricus - genetics; Sulfolobus solfataricus - metabolism; Viruses; Viruses - enzymology; Viruses - genetics; CRISPR; chemical biology; biochemistry; Sulfolobus solfataricus; cyclic oligoadenylate; E. coli; ring nuclease; ribonuclease

Cyclic nucleotide second messengers are increasingly implicated in prokaryotic anti-viral defence systems. Type III CRISPR systems synthesise cyclic oligoadenylate (cOA) upon detecting foreign RNA, activating ancillary nucleases that can be toxic to cells, necessitating mechanisms to remove cOA in s...