Enterocloster alcoholdehydrogenati sp. nov., a Novel Bacterial Species Isolated from the Feces of a Patient with Alcoholism

• Published in: Current microbiology Vol. 80; no. 5; p. 187
• Main Authors: Oikawa, Daiki, Fukui, Kohei, Aoki, Yuichi, Waki, Toshiyuki, Takahashi, Seiji, Shimoyama, Takefumi, Nakayama, Toru
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.05.2023; Springer Nature B.V
• Subjects: Acetaldehyde; Alcoholism; Alcoholism - microbiology; Bacteria; Bacteria - classification; Bacteria - isolation & purification; Biomedical and Life Sciences; Biotechnology; Cell membranes; Chemotaxis; Dimethyl acetals; Fatty acids; Feces; Feces - microbiology; Genomes; Hsp60 protein; Life Sciences; Lipids; Microbiology; New species; Nucleotide sequence; Nucleotides; Phylogeny; RpoB protein; rRNA 16S; Whole Genome Sequencing
• ISSN: 0343-8651; 1432-0991
• DOI: 10.1007/s00284-023-03285-1

Strain C5-48 T , an anaerobic intestinal bacterium that potentially accumulates acetaldehyde at levels exceeding its minimum mutagenic concentration (50 µM) in the colon and rectum, was isolated from the feces of a patient with alcoholism. The 16S rRNA gene sequence of strain C5-48 T showed high similarity to the corresponding sequences of Lachnoclostridium edouardi Marseille-P3397 T (95.7%) and Clostridium fessum SNUG30386 T (94.7%). However, phylogenetic analysis using the sequences of the 16S rRNA, rpoB , and hsp60 genes and whole-genome analysis strongly suggested that C5-48 T should be included in the genus Enterocloster . The novelty of strain C5-48 T was further confirmed by comprehensive average nucleotide identity (ANI) calculations based on its whole-genome sequence, which showed appreciable ANI values with known Enterocloster species ( e.g ., 74.3% and 73.4% with Enterocloster bolteae WAL 16351 T and Enterocloster clostridioformis ATCC 25537 T , respectively). The temperature range for growth of strain C5-48 T was 15–37 °C with an optimum of 37 °C. The pH range for growth was 5.5–10.5 with an optimum of 7.5. The major constituents of the cell membrane lipids of strain C5-48 T were 16:0, 14:0, and 18:1 ω 7 c dimethyl acetal fatty acids. On the basis of the genotypic and phenotypic properties, Enterocloster alcoholdehydrogenati sp. nov. is proposed, with the type strain C5-48 T (= JCM 33305 T  = DSM 109474 T ).