Cytoplasmic contractile injection systems mediate cell death in Streptomyces

• Published in: Nature microbiology Vol. 8; no. 4; pp. 711 - 726
• Main Authors: Casu, Bastien, Sallmen, Joseph W., Schlimpert, Susan, Pilhofer, Martin
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.04.2023; Nature Publishing Group
• Subjects: 101/1; 14/28; 14/34; 14/63; 38/22; 38/23; 631/326/88; 631/535/1258/1260; 82/58; 82/80; 82/83; Apoptosis; Biomedical and Life Sciences; Cell Death; Cell interactions; Contractility; Cryoelectron Microscopy; Cytoplasm; Electron microscopy; Gene clusters; Hyphae; Infectious Diseases; Injection systems; Life Sciences; Light microscopy; Medical Microbiology; Microbiology; Microscopy; Parasitology; Phages; Streptomyces; Streptomyces coelicolor; Streptomyces coelicolor - genetics; Streptomyces coelicolor - metabolism; Virology
• ISSN: 2058-5276; 2058-5276
• DOI: 10.1038/s41564-023-01341-x

Contractile injection systems (CIS) are bacteriophage tail-like structures that mediate bacterial cell–cell interactions. While CIS are highly abundant across diverse bacterial phyla, representative gene clusters in Gram-positive organisms remain poorly studied. Here we characterize a CIS in the Gram-positive multicellular model organism Streptomyces coelicolor and show that, in contrast to most other CIS, S. coelicolor CIS (CIS Sc ) mediate cell death in response to stress and impact cellular development. CIS Sc are expressed in the cytoplasm of vegetative hyphae and are not released into the medium. Our cryo-electron microscopy structure enabled the engineering of non-contractile and fluorescently tagged CIS Sc assemblies. Cryo-electron tomography showed that CIS Sc contraction is linked to reduced cellular integrity. Fluorescence light microscopy furthermore revealed that functional CIS Sc mediate cell death upon encountering different types of stress. The absence of functional CIS Sc had an impact on hyphal differentiation and secondary metabolite production. Finally, we identified three putative effector proteins, which when absent, phenocopied other CIS Sc mutants. Our results provide new functional insights into CIS in Gram-positive organisms and a framework for studying novel intracellular roles, including regulated cell death and life-cycle progression in multicellular bacteria. Characterization of a contractile injection system (CIS) in the Gram-positive multicellular model organism Streptomyces coelicolor finds that CIS mediate cell death in response to stress that impacts cellular development.