Molecular Mechanism of Proinflammatory Cytokine-Mediated Squamous Metaplasia in Human Corneal Epithelial Cells

• Published in: Investigative ophthalmology & visual science Vol. 51; no. 5; pp. 2466 - 2475
• Main Authors: Li, Shimin, Gallup, Marianne, Chen, Ying-Ting, McNamara, Nancy A.
• Format: Journal Article
• Language: English
• Published: United States Association for Research in Vision and Ophthalmology, Inc 01.05.2010
• Subjects: Antigens, Polyomavirus Transforming; Biomarkers; Cell Culture Techniques; Cell Line, Transformed; Cornea - pathology; Cornified Envelope Proline-Rich Proteins - genetics; Cyclic AMP Response Element-Binding Protein - genetics; Cyclic AMP Response Element-Binding Protein - metabolism; Electrophoretic Mobility Shift Assay; Epithelium, Corneal - drug effects; Epithelium, Corneal - metabolism; Epithelium, Corneal - pathology; Extracellular Signal-Regulated MAP Kinases - metabolism; Gene Expression Regulation - physiology; Homeodomain Proteins - genetics; Homeodomain Proteins - metabolism; Humans; Interferon-gamma - pharmacology; Interleukin-1beta - pharmacology; MAP Kinase Kinase 4 - metabolism; Metaplasia; Mutagenesis, Site-Directed; p38 Mitogen-Activated Protein Kinases - metabolism; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - metabolism; Transcription Factors - genetics; Transcription Factors - metabolism; Transfection; Zinc Finger E-box-Binding Homeobox 1

The cornified envelope protein small proline-rich protein 1B (SPRR1B) is a biomarker for squamous metaplasia. Proinflammatory cytokines IL-1beta and IFN-gamma are potent inducers of ocular surface keratinization and SPRR1B expression. Here the molecular mechanisms controlling SPRR1B gene expression...