An integrated platform for high-throughput nanoscopy

• Published in: Nature biotechnology Vol. 41; no. 11; pp. 1549 - 1556
• Main Authors: Barentine, Andrew E. S., Lin, Yu, Courvan, Edward M., Kidd, Phylicia, Liu, Miao, Balduf, Leonhard, Phan, Timy, Rivera-Molina, Felix, Grace, Michael R., Marin, Zach, Lessard, Mark, Rios Chen, Juliana, Wang, Siyuan, Neugebauer, Karla M., Bewersdorf, Joerg, Baddeley, David
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.11.2023; Nature Publishing Group
• Subjects: 631/1647/245; 631/1647/245/2225; 631/61; 631/80/2373/2238; Agriculture; Bioinformatics; Biomedical and Life Sciences; Biomedical Engineering/Biotechnology; Biomedicine; Biotechnology; Data compression; Data processing; Image reconstruction; Imaging, Three-Dimensional; Life Sciences; Localization; Microscopes; Microscopy; Microscopy, Fluorescence - methods; Single Molecule Imaging - methods; Software

Single-molecule localization microscopy enables three-dimensional fluorescence imaging at tens-of-nanometer resolution, but requires many camera frames to reconstruct a super-resolved image. This limits the typical throughput to tens of cells per day. While frame rates can now be increased by over a...