Induction of Metabolism and Transport in Human Intestine: Validation of Precision-Cut Slices as a Tool to Study Induction of Drug Metabolism in Human Intestine in Vitro

• Published in: Drug metabolism and disposition Vol. 36; no. 3; pp. 604 - 613
• Main Authors: VAN DE KERKHOF, Esther G, DE GRAAF, Inge A. M, UNGELL, Anna-Lena B, GROOTHUIS, Geny M. M
• Format: Journal Article
• Language: English
• Published: Bethesda, MD American Society for Pharmacology and Experimental Therapeutics 01.03.2008
• Subjects: Adenosine Triphosphate - metabolism; Biological and medical sciences; Colon - enzymology; Colon - metabolism; Cytochrome P-450 CYP1A1 - biosynthesis; Cytochrome P-450 CYP1A1 - genetics; Cytochrome P-450 CYP3A - biosynthesis; Cytochrome P-450 CYP3A - genetics; Humans; In Vitro Techniques; Jejunum - enzymology; Jejunum - metabolism; Medical sciences; Microtomy - methods; Pharmaceutical Preparations - metabolism; Pharmacology. Drug treatments; Reproducibility of Results; RNA, Messenger - biosynthesis; RNA, Messenger - genetics; Human; Drug; Validation; Digestive system; Induction; Biological transport; Gut; Pharmacokinetics; Metabolism; In vitro

Induction of drug enzyme activity in the intestine can strongly determine plasma levels of drugs. It is therefore important to predict drug-drug interactions in human intestine in vitro. We evaluated the applicability of human intestinal precision-cut slices for induction studies in vitro. Morpholog...