Biochemical fingerprint of colorectal cancer cell lines using label‐free live single‐cell Raman spectroscopy

• Published in: Journal of Raman spectroscopy Vol. 49; no. 8; pp. 1323 - 1332
• Main Authors: Gala de Pablo, Julia, Armistead, Fern J., Peyman, Sally A., Bonthron, David, Lones, Michael, Smith, Stephen, Evans, Stephen D.
• Format: Journal Article
• Language: English
• Published: England Wiley Subscription Services, Inc 01.08.2018; John Wiley and Sons Inc
• Subjects: Adenocarcinoma; Biotechnology; Cancer; Carbohydrates; Chemical fingerprinting; Classification; Colorectal cancer; Colorectal carcinoma; Cytochrome; Cytochrome c; Cytochromes; Deoxyribonucleic acid; Diagnostic software; Diagnostic systems; Discriminant analysis; DNA; Lactic acid; Lipids; living cells; metastasis; Organic chemistry; Phenotyping; Phosphates; Principal components analysis; Protein structure; Proteins; Raman spectra; Raman spectroscopy; Reproducibility; single cell; Spectroscopy; Spectrum analysis; Standard error; Support vector machines; Tumor cell lines; Tumors; living cells; single cell; colorectal cancer; Raman spectroscopy; metastasis

Label‐free live single‐cell Raman spectroscopy was used to obtain a chemical fingerprint of colorectal cancer cells including the classification of the SW480 and SW620 cell line model system, derived from primary and secondary tumour cells from the same patient. High‐quality Raman spectra were acqui...