Differential analysis of animal bone marrow by flow cytometry

• Published in: Cytometry (New York, N.Y.) Vol. 13; no. 6; pp. 638 - 643
• Main Authors: Martin, Ronald A., Brott, David A., Zandee, Joyce C., McKeel, Mildred J.
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 1992; Wiley-Liss
• Subjects: Animals; Biological and medical sciences; Bone Marrow Cells; Bone Marrow Examination - methods; bone marrow indices; Carbocyanines; Cell Count; Cell Division; Cell physiology; Coloring Agents; Dogs; Erythroid maturation index; Female; Flow Cytometry; Fundamental and applied biological sciences. Psychology; Hematopoiesis; Hematopoietic Stem Cells - ultrastructure; laboratory animals; Macaca fascicularis; Male; maturation profile; Mice; Microscopy, Fluorescence; Molecular and cellular biology; myeloid maturation index; myeloid:erythroid ratio; Rats; Rats, Wistar; Cell proliferation; Flow cytometry; Vertebrata; Mammalia; Fluorescent labelling; Cell differentiation; Quantitative analysis
• ISSN: 0196-4763; 1097-0320
• DOI: 10.1002/cyto.990130612

A simple procedure was developed for rapid analysis of animal bone marrow by flow cytometry using the lipophilic cationic dye 3,3′‐dihexyloxacarbocyanine iodide (DiOC6(3)). The batch process allows differentiation of bone marrow cells into lymphoid, erythroid, and myeloid populations and enables classification of erythroid and myeloid cells into proliferating and maturing subpopulations. From these data, myeloid:erythroid (M:E) ratios and maturation indices for erythroid and myeloid cells (EMI and MMI, respectively) can be derived. This procedure provides the opportunity to analyze bone marrow quantitatively and offers distinct advantages to current manual methods in terms of simplicity, throughput, and reproducibility. The method has been tested successfully using marrow from Wistar rats, B6C3F1 mice, beagle dogs, and cynomolgus monkeys. This technique facilitates the evaluation of bone marrow samples taken from preclinical safety studies or from animal colonies of large size. © 1992 Wiley‐Liss, Inc.