Effects of 9‐cis retinoic acid on human homeobox gene NKX3.1 expression in prostate cancer cell line LNCaP

• Published in: Asian journal of andrology Vol. 8; no. 4; pp. 435 - 441
• Main Authors: Jiang, An‐Li, Zhang, Peng‐Ju, Chen, Wei‐Wen, Liu, Wen‐Wen, Yu, Chun‐Xiao, Hu, Xiao‐Yan, Zhang, Xiao‐Qian, Zhang, Jian‐Ye
• Format: Journal Article
• Language: English
• Published: Melbourne, Australia Blackwell Publishing Asia 01.07.2006; Medknow Publications & Media Pvt. Ltd
• Subjects: 9‐cis retinoic acid; Base Sequence; Cell Cycle; Cell Differentiation; Cell Line, Tumor; DNA Primers; Flow Cytometry; gene expression; Gene Expression Regulation, Neoplastic - drug effects; Homeodomain Proteins - genetics; Humans; Male; NKX3.1 gene; Promoter Regions, Genetic; prostate cancer cell; Prostatic Neoplasms - genetics; Prostatic Neoplasms - pathology; Reverse Transcriptase Polymerase Chain Reaction; Transcription Factors - genetics; Tretinoin - pharmacology; Up-Regulation

Aim: To study the regulatory effects of 9‐cis retinoic acid (RA) on the expression of human homeobox gene NKX3.1 in prostate cancer cell line LNCaP. Methods: Flow cytometry, reverse transcriptase polymerase chain reaction and Western blotting were performed to evaluate the effects of 9‐cis RA on NKX...