Baculovirus as vectors for human cells and applications in organ transplantation

• Published in: Journal of invertebrate pathology Vol. 107; pp. S49 - S58
• Main Authors: Hitchman, Richard B, Murguía-Meca, Fernanda, Locanto, Elisabetta, Danquah, John, King, Linda A.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.07.2011
• Subjects: AcMNPV; adaptive immunity; Autographa californica; Autographa californica multiple nucleopolyhedrovirus; Baculovirus; Cells, Cultured; Data processing; Gene Expression Regulation, Viral; Gene therapy; gene transfer; Gene Transfer Techniques; genes; Genetic Therapy - methods; Genetic Vectors; Humans; ischemia; Ischemia reperfusion injury; kidney cells; liver; Nucleopolyhedrovirus - genetics; Organ Transplantation; Reperfusion Injury - genetics; Reperfusion Injury - therapy; RT-PCR profiler array; viruses; AcMNPV; Organ transplantation; Ischemia reperfusion injury; Baculovirus; Gene therapy; RT-PCR profiler array

[Display omitted] ► We discuss baculoviruses as vectors for gene therapy in ischemia reperfusion injury (IRI). ► We test different models of IRI in vitro. ► Baculovirus concentration methods are also compared and the addition of PEG is described. ► Using baculovirus for transduction of solid organs...