In situ structure of virus capsids within cell nuclei by correlative light and cryo-electron tomography

• Published in: Scientific reports Vol. 10; no. 1; p. 17596
• Main Authors: Vijayakrishnan, Swetha, McElwee, Marion, Loney, Colin, Rixon, Frazer, Bhella, David
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 19.10.2020; Nature Publishing Group
• Subjects: 631/1647/245/2160; 631/1647/245/2221; 631/1647/245/2225; 631/326/596/1553; 631/326/88; 631/57/2267; 631/57/2272; 692/699/255/2514; Animals; Capsid - ultrastructure; Capsids; Cell Line; Cell Nucleus - ultrastructure; Cricetinae; Cryoelectron Microscopy; Electron Microscope Tomography; Electron microscopy; Herpesvirus 1, Human - ultrastructure; Humanities and Social Sciences; Microscopy; multidisciplinary; Science; Science (multidisciplinary); Sectioning; Tegument; Tomography; Virion - ultrastructure

Cryo electron microscopy (cryo-EM), a key method for structure determination involves imaging purified material embedded in vitreous ice. Images are then computationally processed to obtain three-dimensional structures approaching atomic resolution. There is increasing interest in extending structur...