Direct In Vivo Manipulation and Imaging of Calcium Transients in Neutrophils Identify a Critical Role for Leading-Edge Calcium Flux

• Published in: Cell reports (Cambridge) Vol. 13; no. 10; pp. 2107 - 2117
• Main Authors: Beerman, Rebecca W., Matty, Molly A., Au, Gina G., Looger, Loren L., Choudhury, Kingshuk Roy, Keller, Philipp J., Tobin, David M.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15.12.2015; Elsevier
• Subjects: Animals; Animals, Genetically Modified; Calcium - metabolism; Calcium Signaling - physiology; Chemotaxis - physiology; Microscopy, Fluorescence - methods; Neutrophils - metabolism; Rats; TRPV Cation Channels - genetics; TRPV Cation Channels - metabolism; Zebrafish

Calcium signaling has long been associated with key events of immunity, including chemotaxis, phagocytosis, and activation. However, imaging and manipulation of calcium flux in motile immune cells in live animals remain challenging. Using light-sheet microscopy for in vivo calcium imaging in zebrafi...