Hyperosmolar blood–brain barrier opening using intra-arterial injection of hyperosmotic mannitol in mice under real-time MRI guidance

• Published in: Nature protocols Vol. 17; no. 1; pp. 76 - 94
• Main Authors: Chu, Chengyan, Jablonska, Anna, Gao, Yue, Lan, Xiaoyan, Lesniak, Wojciech G., Liang, Yajie, Liu, Guanshu, Li, Shen, Magnus, Tim, Pearl, Monica, Janowski, Miroslaw, Walczak, Piotr
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.01.2022; Nature Publishing Group
• Subjects: 631/154/152; 631/1647/245/1628; 631/1647/334/1874/345; 631/378/1341; 692/617; Analytical Chemistry; Animals; Biological Techniques; Biomedical and Life Sciences; Blood-brain barrier; Blood-Brain Barrier - diagnostic imaging; Blood-Brain Barrier - drug effects; Brain damage; Capillary Permeability - drug effects; Carotid arteries; Cerebral blood flow; Chemical compounds; Computational Biology/Bioinformatics; Drug dosages; Injection; Injections, Intra-Arterial; Ischemia; Life Sciences; Magnetic Resonance Imaging; Male; Mannitol; Mannitol - administration & dosage; Mannitol - pharmacology; Medical research; Mice; Mice, SCID; Microarrays; Neurological diseases; Occlusion; Organic Chemistry; Osmotic Pressure; Pharmacology; Protocol; Radiology; Reproducibility; Variability; Veins & arteries

The blood–brain barrier (BBB) is the main obstacle to the effective delivery of therapeutic agents to the brain, compromising treatment efficacy for a variety of neurological disorders. Intra-arterial (IA) injection of hyperosmotic mannitol has been used to permeabilize the BBB and improve parenchym...