Absence of Radial Spokes in Mouse Node Cilia Is Required for Rotational Movement but Confers Ultrastructural Instability as a Trade-Off

• Published in: Developmental cell Vol. 35; no. 2; pp. 236 - 246
• Main Authors: Shinohara, Kyosuke, Chen, Duanduan, Nishida, Tomoki, Misaki, Kazuyo, Yonemura, Shigenobu, Hamada, Hiroshi
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 26.10.2015
• Subjects: Animals; Body Patterning - genetics; Cilia - genetics; Cilia - physiology; Cilia - ultrastructure; Embryo, Mammalian; Embryonic Development - drug effects; Embryonic Development - genetics; Mice; Microtubules - drug effects; Microtubules - ultrastructure; Paclitaxel - administration & dosage

Determination of left-right asymmetry in mouse embryos is established by a leftward fluid flow that is generated by clockwise rotation of node cilia. How node cilia achieve stable unidirectional rotation has remained unknown, however. Here we show that brief exposure to the microtubule-stabilizing d...