Sphingolipid metabolites selectively elicit increases in nuclear calcium concentration in cell suspension cultures and in isolated nuclei of tobacco

• Published in: Cell calcium (Edinburgh) Vol. 43; no. 1; pp. 29 - 37
• Main Authors: Xiong, Tou Cheu, Coursol, Sylvie, Grat, Sabine, Ranjeva, Raoul, Mazars, Christian
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier India Pvt Ltd 01.01.2008; Elsevier
• Subjects: Advanced Basic Science; Aequorin; Calcium - metabolism; Calcium Signaling; Calcium signalling; Cell Fractionation; Cell Nucleus - enzymology; Cell Nucleus - metabolism; Cells, Cultured; Cellular Biology; Life Sciences; Nicotiana - cytology; Nicotiana - enzymology; Nicotiana - metabolism; Phosphotransferases (Alcohol Group Acceptor) - metabolism; Plant cell nucleus; Sphingoid long-chain base; Sphingoid long-chain base kinase; Sphingolipids; Sphingolipids - chemistry; Sphingolipids - metabolism; Sphingolipids - pharmacology; Sphingosine - analogs & derivatives; Sphingosine - chemistry; Sphingosine - pharmacology; Tobacco BY-2 cells; Transient Receptor Potential Channels - antagonists & inhibitors; Calcium signalling; Tobacco BY-2 cells; Sphingolipids; Sphingoid long-chain base kinase; Aequorin; Sphingoid long-chain base; Plant cell nucleus; TRANSPORT DE CALCIUM; PLANT CELL NUCLEUS; TOBACCO BY-2 CELLS; TENEUR EN CALCIUM; SPHINGOID LONG-CHAIN BASE; AEQUORIN; CALCIUM SIGNALLING; SPHINGOLIPIDS; SPHINGOID LONG-CHAIN BASE KINASE

Abstract Sphingolipids are known to interfere with calcium-based signalling pathways. Here we report that these compounds modulate nuclear calcium signalling in tobacco BY-2 cells. Nuclear protein kinase activity phosphorylated endogenous sphingoid long-chain bases (LCBs), suggesting that LCBs are a...