RNA sequencing of corneas from two keratoconus patient groups identifies potential biomarkers and decreased NRF2-antioxidant responses

• Published in: Scientific reports Vol. 10; no. 1; p. 9907
• Main Authors: Shinde, Vishal, Hu, Nan, Mahale, Alka, Maiti, George, Daoud, Yassine, Eberhart, Charles G., Maktabi, Azza, Jun, Albert S., Al-Swailem, Samar A., Chakravarti, Shukti
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 18.06.2020; Nature Publishing Group
• Subjects: 631/1647/2017; 631/208/200; 692/699/3161; 692/699/3161/3163; Adolescent; Adult; African Americans; Aged; Antioxidants; Antioxidants - metabolism; Biomarkers; Biomarkers - metabolism; Calcium-sensing receptors; Case-Control Studies; Cell Cycle Proteins - genetics; Cell Cycle Proteins - metabolism; Cornea; Cornea - metabolism; Cornea - pathology; Extracellular matrix; Gas1 protein; Gene expression; Gene Expression Regulation; Geography; GPI-Linked Proteins - genetics; GPI-Linked Proteins - metabolism; Humanities and Social Sciences; Humans; Immunofluorescence; Keratoconus; Keratoconus - genetics; Keratoconus - metabolism; Keratoconus - pathology; Middle Aged; multidisciplinary; NF-E2-Related Factor 2 - genetics; NF-E2-Related Factor 2 - metabolism; Nucleotide sequence; Oxidants; Oxidative Stress; Oxidizing agents; Pathogenesis; Principal Component Analysis; Principal components analysis; Ribonucleic acid; RNA; RNA - chemistry; RNA - metabolism; Science; Science (multidisciplinary); Sequence Analysis, RNA; Signal transduction; Thrombospondin; Thrombospondins - genetics; Thrombospondins - metabolism; Transcriptomics; Young Adult

Keratoconus is a highly prevalent (1 in 2000), genetically complex and multifactorial, degenerative disease of the cornea whose pathogenesis and underlying transcriptomic changes are poorly understood. To identify disease-specific changes and gene expression networks, we performed next generation RN...