Aster tataricus attenuates asthma efficiently by simultaneously inhibiting tracheal ring contraction and inflammation

• Published in: Biomedicine & pharmacotherapy Vol. 130; p. 110616
• Main Authors: Chen, Yijun, Wu, Hao, Li, Yueting, Liu, Jie, Jia, Zhixin, Xu, Wenjuan, Xiao, Hongbin, Wang, Wei
• Format: Journal Article
• Language: English
• Published: France Elsevier Masson SAS 01.10.2020; Elsevier
• Subjects: Animals; Anti-Asthmatic Agents - pharmacology; Anti-Asthmatic Agents - therapeutic use; Anti-inflammatory activity; Antiasthmatic ability; Aster Plant - chemistry; Aster tataricus; Asthma; Asthma - drug therapy; Asthma - pathology; Bronchoalveolar Lavage Fluid - cytology; Calcium Channel Blockers - pharmacology; Guinea Pigs; Histamine Antagonists - pharmacology; In Vitro Techniques; Lung - pathology; Mice; Muscarinic Antagonists - pharmacology; Muscle Contraction - drug effects; Myocytes, Smooth Muscle - drug effects; Ovalbumin; Plant Extracts - pharmacology; Plant Extracts - therapeutic use; Trachea - drug effects; Trachea - pathology; Tracheitis - drug therapy; Antiasthmatic ability; Aster tataricus; Anti-inflammatory activity; Asthma
• ISSN: 0753-3322; 1950-6007
• DOI: 10.1016/j.biopha.2020.110616

[Display omitted] •RA inhibits the tracheal ring contraction induced by any agonists.•RA decreases the intracellular Ca2+ concentration of tracheal smooth muscle cells.•RA suppresses the inflammatory chemokines and cytokines sensitized by OVA.•RA attenuates asthma via both dilating airway and alleviating lung inflammation. Asthma is an airway chronic inflammatory disease with significant morbidity, mortality and huge social economic burden. Previous research demonstrated that the root of Aster tataricus (RA) may have the potential to treat asthma, but the efficacy and mechanism were not clear. In this study, preliminary results in vitro showed that Fr-75 eluted from RA extract could not only completely inhibit the tracheal ring contraction raised by KCl in 20 min, but also effectively affect the tracheal ring contraction induced by KCl-, Ach- and His in a concentration-dependent manner (3.91−250 μg/mL). Further results on cells exhibited that Fr-75 could decrease the concentration of intracellular Ca2+ as well. These results revealed the underlying mechanism in vitro that the inhibition of tracheal ring contraction might be due to the decline of the intracellular Ca2+ concentration, which caused by suppressing calcium channel, antagonizing the muscarinic and histamine receptors. Also, results in vivo exhibited that Fr-75 could distinctly ease the symptoms of ovalbumin-sensitized mice, including relieving the pathological injury, increasing the latency to preconvulsive dyspnea and to enhanced pause, reducing the inflammatory cells, chemokines and cytokines in BALF and lung tissue. In general, it could be speculated that RA fraction may attenuate asthma through dilating the tracheal ring contraction and alleviating the lung inflammation simultaneously.