Cryopreservation of sessile oak (Quercus petraea (Matt.) Liebl.) plumules using aluminium cryo-plates: influence of cryoprotection and drying

• Published in: Plant methods Vol. 20; no. 1; p. 53
• Main Authors: Wasileńczyk, Urszula, Wawrzyniak, Mikołaj Krzysztof, Martins, João Paulo Rodrigues, Kosek, Paulina, Chmielarz, Paweł
• Format: Journal Article
• Language: English
• Published: England BioMed Central 12.04.2024; BMC
• Subjects: Alginic acid; Aluminium cryoplate; Aluminum; apical meristems; biological safety cabinet; Calcium alginate; Caustic soda; Cryopreservation; cryoprotectants; Cryoprotectors; Desiccants; Desiccation; Drying; encapsulation; Freezing; gels; Gene banks; Generalized linear models; genes; genus; Germplasm; glycerol; Laminar flow; Liquid nitrogen; Long-term storage; Meristems; Moisture content; Nitrogen; Plant germplasm; Plates; plumule; Quercus; Quercus petraea; Regrowth; seed quality; Seeds; Sessile oak; Sodium; species; storage time; Sucrose; Survival; Vitrification; Water shortages; Poland; Europe; Aluminium cryoplate; Sessile oak; Desiccation; Vitrification; Liquid nitrogen; Long-term storage; Micropropagation; In vitro culture; Gene banks; Plant germplasm

BACKGROUND QUERCUS: seeds that are recalcitrant to desiccation and freezing temperatures cannot be stored in gene banks under conventional conditions. However, the germplasm of some recalcitrant seeded species can be stored in liquid nitrogen (-196 °C). Unfortunately, for many species, among them fo...