Supramolecular arrangement of protein in nanoparticle structures predicts nanoparticle tropism for neutrophils in acute lung inflammation

• Published in: Nature nanotechnology Vol. 17; no. 1; pp. 86 - 97
• Main Authors: Myerson, Jacob W., Patel, Priyal N., Rubey, Kathryn M., Zamora, Marco E., Zaleski, Michael H., Habibi, Nahal, Walsh, Landis R., Lee, Yi-Wei, Luther, David C., Ferguson, Laura T., Marcos-Contreras, Oscar A., Glassman, Patrick M., Mazaleuskaya, Liudmila L., Johnston, Ian, Hood, Elizabeth D., Shuvaeva, Tea, Wu, Jichuan, Zhang, Hong-Ying, Gregory, Jason V., Kiseleva, Raisa Y., Nong, Jia, Grosser, Tilo, Greineder, Colin F., Mitragotri, Samir, Worthen, George S., Rotello, Vincent M., Lahann, Joerg, Muzykantov, Vladimir R., Brenner, Jacob S.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.01.2022; Nature Publishing Group
• Subjects: 631/61/350/354; 639/166/985; 639/301/357/537; 639/925/352/152; 639/925/352/2734; Acute Disease; Agglutination - drug effects; Animals; Antibodies - pharmacology; Capillaries; Capsids; Chemistry and Materials Science; Cross-Linking Reagents - chemistry; Crosslinking; Dextrans - chemistry; Diapedesis; Edema; Electrostatic properties; Emergency response; Flow cytometry; Humans; Hydrophobicity; Immunohistochemistry; Inflammation; Inflammation - pathology; Leukocytes (neutrophilic); Lipopolysaccharides; Liposomes; Lung - diagnostic imaging; Lung - pathology; Lungs; Male; Materials Science; Mice; Mice, Inbred C57BL; Muramidase - metabolism; Nanoparticles; Nanoparticles - chemistry; Nanotechnology; Nanotechnology and Microengineering; Neutrophils; Neutrophils - drug effects; Neutrophils - pathology; Opsonin Proteins - metabolism; Opsonization; Proteins; Proteins - chemistry; Respiratory distress syndrome; Selectivity; Static Electricity; Therapeutic applications; Tissue Distribution - drug effects; Tomography, Emission-Computed, Single-Photon; Tomography, X-Ray Computed; Tropism

This study shows that the supramolecular arrangement of proteins in nanoparticle structures predicts nanoparticle accumulation in neutrophils in acute lung inflammation (ALI). We observed homing to inflamed lungs for a variety of nanoparticles with agglutinated protein (NAPs), defined by arrangement...