Metabolic Reprogramming of Cancer-Associated Fibroblasts by IDH3α Downregulation

• Published in: Cell reports (Cambridge) Vol. 10; no. 8; pp. 1335 - 1348
• Main Authors: Zhang, Daoxiang, Wang, Yongbin, Shi, Zhimin, Liu, Jingyi, Sun, Pan, Hou, Xiaodan, Zhang, Jian, Zhao, Shimin, Zhou, Binhua P., Mi, Jun
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 03.03.2015; Elsevier
• Subjects: Animals; Cells, Cultured; Colonic Neoplasms - metabolism; Colonic Neoplasms - pathology; Down-Regulation - drug effects; Electron Transport Complex I - metabolism; Fibroblasts - cytology; Fibroblasts - drug effects; Fibroblasts - metabolism; Glucose - metabolism; Glycolysis - drug effects; Humans; Hypoxia-Inducible Factor 1, alpha Subunit - metabolism; Isocitrate Dehydrogenase - antagonists & inhibitors; Isocitrate Dehydrogenase - genetics; Isocitrate Dehydrogenase - metabolism; Ketoglutaric Acids - metabolism; Metabolic Engineering; Mice; Mice, Inbred BALB C; Mice, Nude; MicroRNAs - metabolism; Oxidative Phosphorylation; Phosphorylation; Platelet-Derived Growth Factor - pharmacology; Prolyl Hydroxylases - metabolism; RNA, Small Interfering - metabolism; Transforming Growth Factor beta1 - pharmacology; Up-Regulation - drug effects

Cancer-associated fibroblasts (CAFs) provide critical metabolites for tumor growth and undergo metabolic reprogramming to support glycolysis. However, the molecular mechanisms responsible for this change remain unclear. Here, we report that TGF-β1- or PDGF-induced CAFs switch from oxidative phosphor...