Antioxidant potentials of furanodihydrobenzoxanthones from Artocarpus elasticus and their protection against oxLDL induced injury in SH-SY5Y cells

• Published in: Biomedicine & pharmacotherapy Vol. 165; p. 115278
• Main Authors: Baiseitova, Aizhamal, Shah, Abdul Bari, Khan, Abdul Majid, Idrees, Muhammad, Kim, Jeong Ho, Lee, Yong Hyun, Kong, Il-Keun, Park, Ki Hun
• Format: Journal Article
• Language: English
• Published: France Elsevier Masson SAS 01.09.2023; Elsevier
• Subjects: Antioxidant; Artocarpus elasticus; Furanodihydrobenzoxanthone; Human neuroblastoma SH-SY5Y cells; LDL oxidation; Furanodihydrobenzoxanthone; Human neuroblastoma SH-SY5Y cells; Artocarpus elasticus; Antioxidant; LDL oxidation
• ISSN: 0753-3322; 1950-6007
• DOI: 10.1016/j.biopha.2023.115278

Exposure to reactive oxygen species (ROS) leads to the oxidation of low-density lipoproteins (LDL), converting them into oxidized ones (oxLDL), which are involved in the pathogenesis of Alzheimer's disease, suggesting a potential link between lipid dysregulation and neurodegenerative processes. Phenolic metabolites derived from Artocarpus elasticus root bark were found to possess significant antioxidant properties at three different radical scavenging assays, including 2,2-diphenyl-1-picrylhydrazyl (DPPH), oxygen radical absorbance capacity (ORAC), and thiobarbituric acid reactive substances (TBARS). Among them, furanodihydrobenzoxanthones (1-3) demonstrated notable protection against Cu2+ induced LDL oxidation, with IC50 values ranging from 0.9 to 2.9 μM in measurement of the malondialdehyde (MDA) production at TBARS and prolonged lag times (>180 min) in the generation of conjugated diene (CD). At a concentration of 10 μM, all three compounds (1-3) effectively protected against LDL oxidation as determined by relative electrophoretic mobility (REM). The most potent compound 1 defended human neuroblastoma SH-SY5Y cells from oxLDL-mediated dysfunction, including oxLDL-induced cytotoxicity, inhibited reactive oxygen species (ROS) formation, and enhancing mitochondrial membrane potential (ΔΨm). Individual components annotation in the ethylacetate extract was performed using LC-ESI-QTOF/MS, which serves as a chemotaxonomic marker for A. elasticus root barks. •Artocarpus elasticus root bark metabolites possess strong antioxidant properties against radicals in various assays.•Furanodihydrobenzoxanthones from A. elasticus root bark protect against Cu2+-induced LDL oxidation.•Artonin W effectively defends SH-SY5Y cells from oxLDL-induced dysfunction and ROS formation.•LC-ESI-TOF/MS identified individual components in ethylacetate extract of A. elasticus root bark.