Identification of astrocyte regulators by nucleic acid cytometry

• Published in: Nature (London) Vol. 614; no. 7947; pp. 326 - 333
• Main Authors: Clark, Iain C., Wheeler, Michael A., Lee, Hong-Gyun, Li, Zhaorong, Sanmarco, Liliana M., Thaploo, Shravan, Polonio, Carolina M., Shin, Seung Won, Scalisi, Giulia, Henry, Amy R., Rone, Joseph M., Giovannoni, Federico, Charabati, Marc, Akl, Camilo Faust, Aleman, Dulce M., Zandee, Stephanie E. J., Prat, Alexandre, Douek, Daniel C., Boritz, Eli A., Quintana, Francisco J., Abate, Adam R.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 09.02.2023; Nature Publishing Group
• Subjects: 38/22; 38/62; 38/90; 38/91; 631/1647/514; 631/250/371; Acids; Animals; Astrocytes; Astrocytes - metabolism; Astrocytes - pathology; Autoimmune diseases; Biomarkers; Cells; Central nervous system; Cytometry; Droplets; Encephalomyelitis, Autoimmune, Experimental; Experimental allergic encephalomyelitis; Gene Editing; Gene expression; Gene Expression Regulation; Gene sequencing; Genomes; Glial cells; Humanities and Social Sciences; Humans; Hydrogels; In vivo methods and tests; Inflammatory diseases; Interrogation; Mice; Mice, Knockout; Microfluidics; Microfluidics - methods; multidisciplinary; Multiple sclerosis; Multiple Sclerosis - pathology; Nervous system; Nucleic acids; Nucleic Acids - analysis; Pathogenesis; Pathology; Perturbation; Science; Science (multidisciplinary); Single-Cell Gene Expression Analysis - methods; Splicing; Surface markers; Target detection; Therapeutic targets; Thermal cycling; Transcription activation; Transcription factors; Transcriptomics

Multiple sclerosis is a chronic inflammatory disease of the central nervous system 1 . Astrocytes are heterogeneous glial cells that are resident in the central nervous system and participate in the pathogenesis of multiple sclerosis and its model experimental autoimmune encephalomyelitis 2 , 3 . Ho...