Frontal affinity chromatography: An excellent method of analyzing weak biomolecular interactions based on a unique principle

• Published in: Biochimica et biophysica acta. General subjects Vol. 1865; no. 1; p. 129761
• Main Author: Kasai, Kenichi
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.01.2021
• Subjects: Animals; Binding Sites; Chromatography, Affinity - instrumentation; Chromatography, Affinity - methods; Equipment Design; Frontal affinity chromatography; Glycocode recognition; Humans; Lectin profiling; Lectins - analysis; Polysaccharides - analysis; Proteins - analysis; Retardation analysis; Weak interaction; Lectin profiling; Frontal affinity chromatography; Glycocode recognition; Retardation analysis; Weak interaction

Not only strong biomolecular interactions but also weak interactions play important roles in ensuring appropriate operations of many biological systems. Although a thorough investigation of the latter is essential in understanding life science, few suitable research tools are available because of in...