Frontal affinity chromatography: An excellent method of analyzing weak biomolecular interactions based on a unique principle

Not only strong biomolecular interactions but also weak interactions play important roles in ensuring appropriate operations of many biological systems. Although a thorough investigation of the latter is essential in understanding life science, few suitable research tools are available because of in...

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Published inBiochimica et biophysica acta. General subjects Vol. 1865; no. 1; p. 129761
Main Author Kasai, Kenichi
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 01.01.2021
Subjects
Animals
Binding Sites
Chromatography, Affinity - instrumentation
Chromatography, Affinity - methods
Equipment Design
Frontal affinity chromatography
Glycocode recognition
Humans
Lectin profiling
Lectins - analysis
Polysaccharides - analysis
Proteins - analysis
Retardation analysis
Weak interaction
Lectin profiling
Frontal affinity chromatography
Glycocode recognition
Retardation analysis
Weak interaction
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Summary:Not only strong biomolecular interactions but also weak interactions play important roles in ensuring appropriate operations of many biological systems. Although a thorough investigation of the latter is essential in understanding life science, few suitable research tools are available because of inherent difficulties. Frontal affinity chromatography (FAC) is a versatile method that overcomes the inherent difficulties to provide accurate information on weak interactions. Since its concept and merit are not widely recognized, a comprehensive interpretation of FAC is provided in this review to encourage its application among researchers. FAC is based on a unique principle of measuring the binding strength by the delayed migration of an analyte through an affinity column. Its utility was elucidated via the lectin-glycan interactions. FAC has a great potential as a research tool to solve many difficult problems in general bioscience that are relevant to almost all researchers. •Frontal affinity chromatography; a powerful analytical tool for weak interactions.•Retardation analysis of molecular interaction based on a unique principle.•One of the most powerful tools for glycobiology, especially in lectin field.
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ISSN:0304-4165
1872-8006
1872-8006
DOI:10.1016/j.bbagen.2020.129761