Circulating microRNAs -192 and -194 are associated with the presence and incidence of diabetes mellitus

• Published in: Scientific reports Vol. 8; no. 1; pp. 14274 - 14
• Main Authors: Jaeger, Andrea, Zollinger, Lukas, Saely, Christoph H., Muendlein, Axel, Evangelakos, Ioannis, Nasias, Dimitris, Charizopoulou, Nikoleta, Schofield, Jonathan D., Othman, Alaa, Soran, Handrean, Kardassis, Dimitris, Drexel, Heinz, Eckardstein, Arnold von
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 24.09.2018; Nature Publishing Group
• Subjects: 38; 38/39; 38/61; 692/163/2743/137/773; 692/53/2423; Aged; AKT2 protein; Animals; Biomarkers; Diabetes; Diabetes mellitus; Diabetes mellitus (insulin dependent); Diabetes mellitus (non-insulin dependent); Diabetes Mellitus, Type 2 - blood; Diabetes Mellitus, Type 2 - genetics; Diabetes Mellitus, Type 2 - pathology; Female; Health risks; Humanities and Social Sciences; Humans; Kaplan-Meier Estimate; Longitudinal studies; Male; Metabolic disorders; Metabolic syndrome; Metabolic Syndrome - blood; Metabolic Syndrome - genetics; Metabolic Syndrome - pathology; Mice; Mice, Knockout; MicroRNAs; MicroRNAs - blood; Middle Aged; miRNA; multidisciplinary; Pilot Projects; Prediabetic State - blood; Prediabetic State - genetics; Prediabetic State - pathology; Proto-Oncogene Proteins c-akt - genetics; Risk factors; Science; Science (multidisciplinary); Serum levels; Akt1 Knockout Mice; MiRNAs; Incident T2DM; Cross-sectional Validation Study; Diabetes Incidence
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-018-32274-9

We sought to identify circulating microRNAs as biomarkers of prevalent or incident diabetes. In a pilot study of 18 sex- and age-matched patients with metabolic syndrome, nine of whom developed diabetes during 6 years of follow-up, an array of 372 microRNAs discovered significantly elevated serum levels of microRNAs -122, -192, -194, and -215 in patients who developed diabetes mellitus type 2 (T2DM). In two cross-sectional validation studies, one encompassing sex- and age-matched groups of patients with T2DM, impaired fasting glucose (IFG) and euglycemic controls (n = 43 each) and the other 53 patients with type 1 diabetes and 54 age- and BMI-matched euglycemic controls, serum levels of miR-192, miR-194, and mi215 were significantly higher in diabetic subjects than in probands with euglycemia or IFG. In a longitudinal study of 213 initially diabetes-free patients of whom 35 developed diabetes during 6 years of follow-up, elevated serum levels of microRNAs 192 and 194 were associated with incident T2DM, independently of fasting glucose, HbA1c and other risk factors. Serum levels of miR-192 and miR-194 were also elevated in diabetic Akt2 knockout mice compared to wild type mice. In conclusion, circulating microRNAs -192 and -194 are potential biomarkers for risk of diabetes.