Cytosolic Pellino-1-Mediated K63-Linked Ubiquitination of IRF5 in M1 Macrophages Regulates Glucose Intolerance in Obesity

• Published in: Cell reports (Cambridge) Vol. 20; no. 4; pp. 832 - 845
• Main Authors: Kim, Donghyun, Lee, Ho, Koh, Jaemoon, Ko, Jae Sung, Yoon, Bo Ruem, Jeon, Yoon Kyung, Cho, Young Min, Kim, Tae Han, Suh, Yun-Suhk, Lee, Hyuk-Joon, Yang, Han-Kwang, Park, Kyong Soo, Kim, Hye Young, Lee, Chang Woo, Lee, Won-Woo, Chung, Doo Hyun
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 25.07.2017; Elsevier
• Subjects: Animals; Chromatin Immunoprecipitation; cytosolic mediator; Female; Flow Cytometry; Glucose Intolerance - genetics; Glucose Intolerance - metabolism; glucose metabolism; Humans; Immunoblotting; Immunoprecipitation; Interferon Regulatory Factors - genetics; Interferon Regulatory Factors - metabolism; IRF5; M1 polarization; macrophages; Macrophages - metabolism; Male; Mice; Mice, Knockout; Nuclear Proteins - genetics; Nuclear Proteins - metabolism; obesity; Obesity - genetics; Obesity - metabolism; Pellino-1; Signal Transduction - genetics; Signal Transduction - physiology; ubiquitin E3 ligase; Ubiquitin-Protein Ligases - genetics; Ubiquitin-Protein Ligases - metabolism; ubiquitination; Ubiquitination - genetics; Ubiquitination - physiology; macrophages; ubiquitination; ubiquitin E3 ligase; Pellino-1; IRF5; M1 polarization; glucose metabolism; cytosolic mediator; obesity
• ISSN: 2211-1247; 2211-1247
• DOI: 10.1016/j.celrep.2017.06.088

IRF5 is a signature transcription factor that induces M1 macrophage polarization. However, little is known regarding cytosolic proteins that induce IRF5 activation for M1 polarization. Here, we report the interaction between ubiquitin E3 ligase Pellino-1 and IRF5 in the cytoplasm, which increased nu...