LDAF1 and Seipin Form a Lipid Droplet Assembly Complex

• Published in: Developmental cell Vol. 51; no. 5; pp. 551 - 563.e7
• Main Authors: Chung, Jeeyun, Wu, Xudong, Lambert, Talley J., Lai, Zon Weng, Walther, Tobias C., Farese, Robert V.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 02.12.2019
• Subjects: Berardinelli-Seip congenital lipodystrophy type 2; Cell Line, Tumor; endoplasmic reticulum; Endoplasmic Reticulum - metabolism; fat; GTP-Binding Protein gamma Subunits - metabolism; HEK293 Cells; Humans; lipid droplets; Lipid Droplets - metabolism; lipodystrophy; Membrane Proteins - metabolism; organelle biogenesis; promethin; Protein Binding; TMEM159; triacylglycerol; Triglycerides - metabolism; triacylglycerol; TMEM159; fat; organelle biogenesis; Berardinelli-Seip congenital lipodystrophy type 2; lipid droplets; endoplasmic reticulum; lipodystrophy; promethin

Lipid droplets (LDs) originate from the endoplasmic reticulum (ER) to store triacylglycerol (TG) and cholesterol esters. The ER protein seipin was shown to localize to ER-LD contacts soon after LDs form, but what determines the sites of initial LD biogenesis in the ER is unknown. Here, we identify T...