Variable levels of spike and ORF1ab RNA in post-mortem lung samples of SARS-CoV-2-positive subjects: comparison between ISH and RT-PCR

• Published in: Virchows Archiv : an international journal of pathology Vol. 480; no. 3; pp. 597 - 607
• Main Authors: Zito Marino, Federica, De Cristofaro, Tiziana, Varriale, Massimo, Zannini, Giuseppa, Ronchi, Andrea, La Mantia, Elvira, Campobasso, Carlo Pietro, De Micco, Francesco, Mascolo, Pasquale, Municinò, Maurizio, Municinò, Emilia, Vestini, Francesco, Pinto, Omero, Moccia, Marta, De Stefano, Noè, Nappi, Oscar, Sementa, Carmen, Zotti, Giovanni, Pianese, Lamberto, Giordano, Carmela, Franco, Renato
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.03.2022; Springer Nature B.V
• Subjects: Amplification; COVID-19; COVID-19 - diagnosis; Electron microscopy; Gene amplification; Gene sequencing; Humans; Hybridization; Immunohistochemistry; In Situ Hybridization; Lung; Lungs; Medicine; Medicine & Public Health; N gene; Nucleocapsids; Optimization; Original; Original Article; Paraffin; Paraffins; Pathology; Polymerase chain reaction; Reverse Transcriptase Polymerase Chain Reaction; Reverse transcription; Ribonucleic acid; RNA; RNA, Viral - genetics; S gene; SARS-CoV-2 - genetics; Sensitivity and Specificity; Severe acute respiratory syndrome coronavirus 2; Viral diseases; Viral envelope proteins; Viruses; Reverse transcription polymerase chain reaction (RT-PCR); SARS-CoV-2; Spike(S); Open reading frame (ORF1ab); Post-mortem lung samples; In situ hybridization (ISH)

Post-mortem examination plays a pivotal role in understanding the pathobiology of the SARS-CoV-2; thus, the optimization of virus detection on the post-mortem formalin-fixed paraffin-embedded (FFPE) tissue is needed. Different techniques are available for the identification of the SARS-CoV-2, includ...