Evaluating ribosomal frameshifting in CCR5 mRNA decoding

• Published in: Nature (London) Vol. 604; no. 7906; pp. E16 - E23
• Main Authors: Khan, Yousuf A., Loughran, Gary, Steckelberg, Anna-Lena, Brown, Katherine, Kiniry, Stephen J., Stewart, Hazel, Baranov, Pavel V., Kieft, Jeffrey S., Firth, Andrew E., Atkins, John F.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 21.04.2022; Nature Publishing Group
• Subjects: 38/39; 631/337/574/1789; 631/45/500; Amino acid sequence; Amino acids; CCR5 protein; Complementary DNA; Efficiency; Frameshifting, Ribosomal - genetics; Gene expression; Humanities and Social Sciences; Laboratories; Matters Arising; multidisciplinary; Nucleic Acid Conformation; Polymerase chain reaction; Proteins; Reticulocytes; Reverse transcription; Ribosomes - genetics; Ribosomes - metabolism; RNA polymerase; RNA, Messenger - genetics; RNA, Messenger - metabolism; Science; Science (multidisciplinary)

PCR with reverse transcription (RT-PCR) of reporter RNAs showed that for cells transfected with the CCRS reporters, there was substantially less full-length cDNA and a simultaneous appearance of smaller molecular mass products (Fig. 1g). [...]the claim that the frameshift site is highly conserved am...